肿瘤抑制因子miR-520a通过负调控急性髓系白血病PI3K/AKT信号通路抑制细胞生长。

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Jing Xiao, Fang Wan, Lin Tian, Yao Li
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引用次数: 0

摘要

背景:短调控RNA,称为微小RNA(miRNA),已被发现在癌症中具有调控功能,因此,最近已被评估其对各种人类恶性肿瘤的治疗作用。目的:本研究旨在探讨miR-520a是否能在人类急性髓系白血病的治疗中发挥作用。材料和方法:使用人髓系白血病细胞系(Kasumi-1、Kasumi-3、Kasumi-6、BDCM和K562)和正常髓系细胞系(NCI-H5N6)进行研究。对细胞系进行实时定量聚合酶链式反应(RT-qPCR),通过MTT测定和集落形成测定评估细胞活力和增殖。用miR-阴性对照(NC)或miR-520a模拟物对转染的K562细胞进行双吖啶橙(AO)/溴化乙锭(EB)染色,并进行膜联蛋白V-异硫氰酸荧光素(FITC)/碘化丙锭(PI)双染色和流式细胞术分析癌症细胞凋亡,然后进行蛋白质印迹。结果:癌细胞系中miR-520a基因表达较低,其过度表达显著降低了癌症细胞的增殖能力(p<0.05)。癌症细胞在miR-520a过度表达后表现出Bax/Bcl-2介导的凋亡诱导。miR-520a在人类急性髓系白血病中靶向PI3K/AKT信号通路,以发挥其在癌症中的调节作用。结论:该研究表明,miR-520a在急性髓系白血病中积极调节细胞增殖,并阐明了其发挥调节作用的机制,强调了靶向miR-520a作为对抗人类急性髓细胞白血病的有效治疗策略的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tumor suppressor miR-520a inhibits cell growth by negatively regulating PI3K/AKT signaling pathway in acute myeloid leukemia.

Background: Short regulatory RNAs, called microRNAs (miRNAs), have been found to possess regulatory functions in cancer and, as such, have recently been evaluated for their therapeutic role against various human malignancies.

Objectives: The present work aimed to investigate whether miR-520a can play a therapeutic role in the treatment of human acute myeloid leukemia.

Material and methods: Human myeloid leukemia cell lines (Kasumi-1, Kasumi-3, Kasumi-6, BDCM, and K562) and a normal myeloid cell line (NCI-H5N6) were used for the study. Cell lines were subjected to real-time quantitative polymerase chain reaction (RT-qPCR), evaluation of cell viability and proliferation by MTT assay and colony formation assays. Dual acridine orange (AO)/ethidium bromide (EB) staining was applied for transfected K562 cells with miR-negative control (NC) or miR-520a mimics, and annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual staining and flow cytometry were performed to analyze cancer cell apoptosis followed by western blot.

Results: Cancerous cell lines exhibited lower gene expression of miR-520a, and its overexpression significantly reduced (p < 0.05) the proliferation and viability of cancer cells. Cancer cells demonstrated the induction of Bax/Bcl-2-mediated apoptosis following miR-520a overexpression. The miR-520a was shown to target the PI3K/AKT signaling pathway in human acute myeloid leukemia to exercise its regulatory role in cancer.

Conclusions: The study showed that miR-520a actively regulated cell proliferation in acute myeloid leukemia and illustrated the mechanism by which it exerts its regulatory role, emphasizing the possibility of targeting miR-520a as an efficient therapeutic strategy against human acute myeloid leukemia.

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来源期刊
Advances in Clinical and Experimental Medicine
Advances in Clinical and Experimental Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
3.70
自引率
4.80%
发文量
153
审稿时长
6-12 weeks
期刊介绍: Advances in Clinical and Experimental Medicine has been published by the Wroclaw Medical University since 1992. Establishing the medical journal was the idea of Prof. Bogumił Halawa, Chair of the Department of Cardiology, and was fully supported by the Rector of Wroclaw Medical University, Prof. Zbigniew Knapik. Prof. Halawa was also the first editor-in-chief, between 1992-1997. The journal, then entitled "Postępy Medycyny Klinicznej i Doświadczalnej", appeared quarterly. Prof. Leszek Paradowski was editor-in-chief from 1997-1999. In 1998 he initiated alterations in the profile and cover design of the journal which were accepted by the Editorial Board. The title was changed to Advances in Clinical and Experimental Medicine. Articles in English were welcomed. A number of outstanding representatives of medical science from Poland and abroad were invited to participate in the newly established International Editorial Staff. Prof. Antonina Harłozińska-Szmyrka was editor-in-chief in years 2000-2005, in years 2006-2007 once again prof. Leszek Paradowski and prof. Maria Podolak-Dawidziak was editor-in-chief in years 2008-2016. Since 2017 the editor-in chief is prof. Maciej Bagłaj. Since July 2005, original papers have been published only in English. Case reports are no longer accepted. The manuscripts are reviewed by two independent reviewers and a statistical reviewer, and English texts are proofread by a native speaker. The journal has been indexed in several databases: Scopus, Ulrich’sTM International Periodicals Directory, Index Copernicus and since 2007 in Thomson Reuters databases: Science Citation Index Expanded i Journal Citation Reports/Science Edition. In 2010 the journal obtained Impact Factor which is now 1.179 pts. Articles published in the journal are worth 15 points among Polish journals according to the Polish Committee for Scientific Research and 169.43 points according to the Index Copernicus. Since November 7, 2012, Advances in Clinical and Experimental Medicine has been indexed and included in National Library of Medicine’s MEDLINE database. English abstracts printed in the journal are included and searchable using PubMed http://www.ncbi.nlm.nih.gov/pubmed.
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