九藻发酵产胰高血糖素伯克霍尔德菌B1213深层发酵条件的优化

IF 0.7 4区 农林科学 Q3 AGRONOMY
Cheng Ruiwen, Wang Fuqiang, Xu Yiren, Wei Lai, Ma Jinghao, Gao Peng, Yang Ran, Liu Xiaoyan, Fan Guangsen
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引用次数: 0

摘要

以白酒蒸馏残渣酒糟为碳源,优化了产胰高血糖素酶的发酵条件。通过单因素设计,分别研究了酒藻颗粒大小和浓度、氮源类型、尿素浓度、初始pH、接种物大小、装载量、振荡速度、温度、表面活性剂类型和培养时间对毕赤酵母B1213产葡聚糖酶的影响。然后,使用Plackett-Burman设计,发现酒藻浓度、接种物大小、初始pH、温度和培养时间五个变量显著影响葡聚糖酶的产生。随后,使用最陡的上升路径和响应面方法设计,找到了由Pyrocinia B.B1213生产葡聚糖酶的最佳条件:粒度,40-60目;酒藻浓度58.4g/L;尿素浓度,8g/L;初始pH,6;加载体积,15mL/250mL;接种量0.63%(v/v);温度,26°C;摇动速度,160转/分;培养时间120h。在这些条件下,P.pyrcinia B1213菌株的葡聚糖酶活性为1336U/mL,由副产物产生白酒酿造所需的生物酶。本研究为在白酒生产中应用牛瘟菌B1213提供了实验数据和理论依据。关键词:pyrocinia伯克霍尔德菌;葡聚糖酶;响应面方法;发酵条件;酒造
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of submerged fermentation conditions for glucanase production by Burkholderia pyrrocinia B1213 using Jiuzao
This study optimized the fermentation conditions for producing glucanase from Burkholderia pyrrocinia B1213 using Jiuzao, a residue from Baijiu distillation, as the carbon source. The effects of Jiuzao particle size and concentration, type of nitrogen source, urea concentration, initial pH, inoculum size, loading volume, shaking speed, temperature, surfactant type and incubation time on glucanase production by B. pyrrocinia B1213 were investigated separately through single factor design. Then, five variables, Jiuzao concentration, inoculum size, initial pH, temperature and incubation time, were found to significantly affect glucanase production using the Plackett-Burman design. Following, the optimal conditions for glucanase production by B. pyrrocinia B1213 were found using the steepest ascent path and response surface methodology designs as: particle size, 40-60 mesh; Jiuzao concentration, 58.4 g/L; urea concentration, 8 g/L; initial pH, 6; loading volume, 15 mL/250 mL; inoculum size, 0.63% (v/v); temperature, 26 °C; shaking speed, 160 rpm; and incubation time, 120 h. Under these conditions, the glucanase activity of the B. pyrrcinia B1213 strain was 1336 U/mL, producing the biological enzymes needed in Baijiu making from a by-product. This study has provided experimental data and theoretical information for using B. pyrrcinia B1213 in Baijiu production. Keywords: Burkholderia pyrrocinia; Glucanase; Response surface methodology; Fermentation conditions; Jiuzao
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来源期刊
Emirates Journal of Food and Agriculture
Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY
CiteScore
1.80
自引率
0.00%
发文量
18
期刊介绍: The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.
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