Ghrelin对小鼠创伤性脑损伤的神经保护作用

中华行为医学与脑科学杂志 Pub Date : 2019-11-20 DOI:10.3760/CMA.J.ISSN.1674-6554.2019.11.004

Xuefei Shao, Lei Zhu, T. Yi, Bo Li, Shi-Xiang Cheng

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The changes of cerebral blood perfusion (CBP) were monitored in real time by laser speckle contrast analysis(LSCI), the changes of neuroelectrophysiology were observed by monitoring motor evoked potential (MEP), and the status of neurological deficit was evaluated by modified neurological deficit score (mNSS). \n \n \nResults \nCompared with Sham group, the mice in TBI group had significantly lower cerebral blood perfusion(CBP) (t=-12.36, P<0.01), longer latency and lower amplitude of motor evoked potential (MEP) (t=5.03, -11.55, all P<0.01), and significantly higher mNSS scores (t=9.34, P<0.01). However, compared with the TBI group, the cerebral blood perfusion(CBP) of Ghrelin group increased significantly at 12 h after TBI((196.87±17.36) PU/mm2vs (123.62±8.04)PU/mm2, t=10.45, P<0.01), while the latency of MEP decreased((5.30±0.33)ms vs (6.80±0.97)ms, t=-5.01, P<0.01), the amplitude of MEP increased((2.21±0.16)mV vs (1.27±0.27)mV, t=9.65, P<0.01). 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引用次数: 0

摘要

目的探讨Ghrelin对小鼠创伤性脑损伤（TBI）的神经保护作用。方法采用电子皮层冲击仪（eCCI）建立C57BL/6小鼠TBI模型。根据随机数字表法，将24只小鼠随机分为假手术组（假手术组）、TBI组和Ghrelin干预组（Ghrelin组），每组8只。TBI组和Ghrelin组建立TBI模型。Ghrelin组小鼠分别于损伤前和损伤后1h腹膜内注射0.5g/kg。Sham组和TBI组分别注射等量生理盐水。通过激光散斑对比分析（LSCI）实时监测脑血流灌注（CBP）的变化，通过监测运动诱发电位（MEP）观察神经电生理学的变化，并通过改良神经功能缺损评分（mNSS）评估神经功能缺损的状态。结果与Sham组相比，TBI组小鼠脑血流灌注（CBP）显著降低（t=-12.36，P<0.01），运动诱发电位（MEP）潜伏期和波幅延长（t=5.03，-11.55，均P<0.01），mNSS评分显著升高（t=9.34，P<0.01），Ghrelin组脑血流灌注（CBP）在TBI后12h显著增加（（196.87±17.36）PU/mm2vs（123.62±8.04）PU/mm2，t=10.45，P<0.01），MEP潜伏期降低（（5.30±0.33）ms vs（6.80±0.97）ms，t=-5.01，P<0.01）、MEP幅度增加（（2.21±0.16）mV vs（1.27±0.27）mV，t=9.65，P<0.01，Ghrelin组在TBI后24 h神经功能缺损评分显著下降（（4.9±1.2）vs（8.4±2.6），t=-3.87，P<0.01）。关键词：创伤性脑损伤；Ghrelin；脑血流灌注；运动诱发电位；神经保护；老鼠

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Neuroprotective effects of Ghrelin on traumatic brain injury in mice

Objective To investigate the neuroprotective effect of Ghrelin on traumatic brain injury (TBI) in mice. Methods TBI model of C57BL / 6 mice was established by electronic cortical impact instrument (eCCI). According to the random figure table method, twenty-four mice were randomly divided into sham group(Sham group), TBI group and Ghrelin intervention group(Ghrelin group) with 8 mice in each group. The model of TBI was established in TBI group and Ghrelin group.The mice in Ghrelin group was injected intraperitoneally 0.5 g/kg before and 1 h after injury respectively. And the mice Sham group and TBI group were injected with the same amount of normal saline. The changes of cerebral blood perfusion (CBP) were monitored in real time by laser speckle contrast analysis(LSCI), the changes of neuroelectrophysiology were observed by monitoring motor evoked potential (MEP), and the status of neurological deficit was evaluated by modified neurological deficit score (mNSS). Results Compared with Sham group, the mice in TBI group had significantly lower cerebral blood perfusion(CBP) (t=-12.36, P<0.01), longer latency and lower amplitude of motor evoked potential (MEP) (t=5.03, -11.55, all P<0.01), and significantly higher mNSS scores (t=9.34, P<0.01). However, compared with the TBI group, the cerebral blood perfusion(CBP) of Ghrelin group increased significantly at 12 h after TBI((196.87±17.36) PU/mm2vs (123.62±8.04)PU/mm2, t=10.45, P<0.01), while the latency of MEP decreased((5.30±0.33)ms vs (6.80±0.97)ms, t=-5.01, P<0.01), the amplitude of MEP increased((2.21±0.16)mV vs (1.27±0.27)mV, t=9.65, P<0.01). And compared with the TBI group, the neurological deficit score of Ghrelin group decreased significantly at 24 h after TBI((4.9±1.2) vs (8.4±2.6), t=-3.87, P<0.01). Conclusion Ghrelin exhibits a significant neuroprotective role by increasing cerebral blood flow perfusion, reducing the degree of neurological deficit and promoting motor function recovery in TBI mice. Key words: Traumatic brain injury; Ghrelin; Cerebral blood perfusion; Motor evoked potential; Neuroprotection; Mice

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中华行为医学与脑科学杂志

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期刊介绍： "Chinese Journal of Behavioral Medicine and Brain Science" (CN 37-1468/R, ISSN 1674-6554) is a national academic journal under the supervision of the National Health Commission, sponsored by the Chinese Medical Association and Jining Medical College. The journal was founded in June 1992 and was formerly known as "Chinese Journal of Behavioral Medicine" (1992-1993) and "Chinese Behavioral Medical Science" (1994-2008). In 2009, it was renamed "Chinese Journal of Behavioral Medicine and Brain Science" with the approval of the State Administration of Press, Publication, Radio, Film and Television. The purpose of "Chinese Journal of Behavioral Medicine and Brain Science" is to implement the health and health policies of the Party and the State, implement the principle of combining theory with practice and popularization and improvement, and reflect the major progress in the theory and practical application of behavioral medicine and brain science in my country. It publishes academic papers and scientific research results in the field of behavioral medicine and brain science in my country, and has columns such as monographs/reviews, basic research, clinical research, health prevention, methods and techniques, psychological behavior and evaluation, and systematic evaluation.