BanLec对1型和2型单纯疱疹病毒的体外抗病毒活性

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2020-03-08 DOI:10.3329/bjp.v15i1.42320

Arisha Taj Mahaboob Batcha, A. Wadhwani, Gowri Subramaniam

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引用次数: 5

摘要

本研究评估了香蕉凝集素（BanLec）对单纯疱疹病毒1型和2型（HSV-1和HSV-2）的抗病毒活性。凝集素是从成熟的香蕉果肉中分离出来的。研究表明，凝集素对人红细胞A、B、AB和O组具有血凝活性。使用SDS凝胶电泳发现BanLec的分子量为14000-3000Da。BanLec对Vero细胞系的细胞毒性显示出172.7µg/mL的抑制浓度。BanLec是杀病毒的，在测试的浓度下没有显示出细胞毒性。凝集素显示出剂量依赖性抗病毒活性，抑制HSV-1 16.0µg/mL，选择性指数10.8，抑制HSV-2 67.7µg/mL。这些结果证实，与HSV-2相比，BanLec可能是HSV-1潜在抗病毒化合物的丰富来源。

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In vitro antiviral activity of BanLec against herpes simplex viruses type 1 and 2

The present study evaluates the antiviral activity of banana lectin (BanLec) against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2). Lectin was isolated from the ripen pulp of bananas (Musa paradisiaca). The study showed that lectin exhibited hemagglutination activity towards human erythrocytes A, B, AB and O group. The molecular weight of BanLec using SDS gel-electrophoresis was found to be 14,000-30,000 Da. Cytotoxicity of BanLec on the Vero cell lines showed an inhibitory concentration of 172.7 µg/mL. BanLec was virucidal and showed no cytotoxicity at the concentration tested. The lectin showed a dose-dependent antiviral activities, inhibiting HSV-1 by 16.0 µg/mL with selectivity index 10.8 and HSV-2 inhibition by 67.7 µg/mL with selectivity index 2.6. These results corroborate that BanLec could be a rich source of potential antiviral compound for HSV-1 when compared to HSV-2.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.