L-肉碱联合表阿霉素对不同肺腺癌细胞增殖和凋亡的影响

Yintao Ye, W. Zhong, Junqiang Qian, Yun-Wei Shi, Chen Wang
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引用次数: 0

摘要

目的探讨L-肉碱(LCNT)联合表阿霉素(EPI)对两种肺腺癌细胞株(GLC-82和A549)增殖和凋亡的影响。方法将GLC-82和A549细胞分别分为对照组、EPI组、LCNT组和EPI+LCNT组。MTT法检测细胞增殖率,流式细胞仪分析细胞周期和细胞早期凋亡。Western Blot法检测P53和Bcl-2蛋白的表达。结果与对照组相比,在20.00μg/ml LCNT处理24小时后,GLC-82和A549细胞的增殖率为37.56%(P<0.05),表明LCNT能促进GLC-82细胞的增殖。与EPI组相比,EPI+LCNT对GLC-82细胞增殖的抑制作用较小,其抑制率比EPI组低12.14%(P<0.05)。LCNT可促进GLC-82细胞增殖,阻断G0/G1期细胞周期。这种机制可能与P53蛋白的下调和Bcl-2蛋白表达的上调有关。关键词:肺肿瘤;腺癌;细胞增殖;细胞凋亡;左旋肉碱;表阿霉素
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of cell proliferation and apoptosis of L-carnitine combined with epirubicin on different human lung adenocarcinoma cells
Objective To investigate the effect of L-carnitine (LCNT) combined with epirubicin (EPI) on cell proliferation and apoptosis of two lung adenocarcinoma cell lines (GLC-82 and A549). Methods GLC-82 and A549 cells were divided into control group, EPI group, LCNT group and EPI+LCNT group, respectively. The cell proliferation rate was examined by MTT assay 24 h after the treatment, and the cell cycle and cell early apoptosis were analyzed by flow cytometry. The expression of P53 and Bcl-2 proteins were detected by Western Blot. Results Compared with the control group, the proliferation rate of GLC-82 and A549 cells was 37.56%(P 0.05) after 24 hours of 20.00 μg/ml LCNT treatment indicating LCNT could promote the proliferation of GLC-82 cells. Compared with the EPI group, EPI+LCNT had smaller inhibitory effect on the proliferation of GLC-82 cells, and the inhibition rate of the EPI+LCNT group was 12.14% lower than that of EPI group (P 0.05). Conclusions Comparing with the EPI, the combination of LCNT and EPI has less proliferation inhibition and apoptosis induction on GLC-82 cells, and without significant effect on A549 cells. LCNT can promote the proliferation of GLC-82 cells and block the cell cycle at G0/G1 phase. This mechanism may be related to down-regulation of P53 protein and up-regulation of Bcl-2 protein expression. Key words: Lung neoplasms; Adenocarcinoma; Cell proliferation; Cell apoptosis; L-carnitine; Epirubicin
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