L-肉碱联合表阿霉素对不同肺腺癌细胞增殖和凋亡的影响

Yintao Ye, W. Zhong, Junqiang Qian, Yun-Wei Shi, Chen Wang
{"title":"L-肉碱联合表阿霉素对不同肺腺癌细胞增殖和凋亡的影响","authors":"Yintao Ye, W. Zhong, Junqiang Qian, Yun-Wei Shi, Chen Wang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.01.003","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effect of L-carnitine (LCNT) combined with epirubicin (EPI) on cell proliferation and apoptosis of two lung adenocarcinoma cell lines (GLC-82 and A549). \n \n \nMethods \nGLC-82 and A549 cells were divided into control group, EPI group, LCNT group and EPI+LCNT group, respectively. The cell proliferation rate was examined by MTT assay 24 h after the treatment, and the cell cycle and cell early apoptosis were analyzed by flow cytometry. The expression of P53 and Bcl-2 proteins were detected by Western Blot. \n \n \nResults \nCompared with the control group, the proliferation rate of GLC-82 and A549 cells was 37.56%(P 0.05) after 24 hours of 20.00 μg/ml LCNT treatment indicating LCNT could promote the proliferation of GLC-82 cells. Compared with the EPI group, EPI+LCNT had smaller inhibitory effect on the proliferation of GLC-82 cells, and the inhibition rate of the EPI+LCNT group was 12.14% lower than that of EPI group (P 0.05). \n \n \nConclusions \nComparing with the EPI, the combination of LCNT and EPI has less proliferation inhibition and apoptosis induction on GLC-82 cells, and without significant effect on A549 cells. LCNT can promote the proliferation of GLC-82 cells and block the cell cycle at G0/G1 phase. This mechanism may be related to down-regulation of P53 protein and up-regulation of Bcl-2 protein expression. \n \n \nKey words: \nLung neoplasms; Adenocarcinoma; Cell proliferation; Cell apoptosis; L-carnitine; Epirubicin","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of cell proliferation and apoptosis of L-carnitine combined with epirubicin on different human lung adenocarcinoma cells\",\"authors\":\"Yintao Ye, W. Zhong, Junqiang Qian, Yun-Wei Shi, Chen Wang\",\"doi\":\"10.3760/CMA.J.ISSN.1673-4181.2019.01.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effect of L-carnitine (LCNT) combined with epirubicin (EPI) on cell proliferation and apoptosis of two lung adenocarcinoma cell lines (GLC-82 and A549). \\n \\n \\nMethods \\nGLC-82 and A549 cells were divided into control group, EPI group, LCNT group and EPI+LCNT group, respectively. The cell proliferation rate was examined by MTT assay 24 h after the treatment, and the cell cycle and cell early apoptosis were analyzed by flow cytometry. The expression of P53 and Bcl-2 proteins were detected by Western Blot. \\n \\n \\nResults \\nCompared with the control group, the proliferation rate of GLC-82 and A549 cells was 37.56%(P 0.05) after 24 hours of 20.00 μg/ml LCNT treatment indicating LCNT could promote the proliferation of GLC-82 cells. Compared with the EPI group, EPI+LCNT had smaller inhibitory effect on the proliferation of GLC-82 cells, and the inhibition rate of the EPI+LCNT group was 12.14% lower than that of EPI group (P 0.05). \\n \\n \\nConclusions \\nComparing with the EPI, the combination of LCNT and EPI has less proliferation inhibition and apoptosis induction on GLC-82 cells, and without significant effect on A549 cells. LCNT can promote the proliferation of GLC-82 cells and block the cell cycle at G0/G1 phase. This mechanism may be related to down-regulation of P53 protein and up-regulation of Bcl-2 protein expression. \\n \\n \\nKey words: \\nLung neoplasms; Adenocarcinoma; Cell proliferation; Cell apoptosis; L-carnitine; Epirubicin\",\"PeriodicalId\":61751,\"journal\":{\"name\":\"国际生物医学工程杂志\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"国际生物医学工程杂志\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.01.003\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"国际生物医学工程杂志","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.01.003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的探讨L-肉碱(LCNT)联合表阿霉素(EPI)对两种肺腺癌细胞株(GLC-82和A549)增殖和凋亡的影响。方法将GLC-82和A549细胞分别分为对照组、EPI组、LCNT组和EPI+LCNT组。MTT法检测细胞增殖率,流式细胞仪分析细胞周期和细胞早期凋亡。Western Blot法检测P53和Bcl-2蛋白的表达。结果与对照组相比,在20.00μg/ml LCNT处理24小时后,GLC-82和A549细胞的增殖率为37.56%(P<0.05),表明LCNT能促进GLC-82细胞的增殖。与EPI组相比,EPI+LCNT对GLC-82细胞增殖的抑制作用较小,其抑制率比EPI组低12.14%(P<0.05)。LCNT可促进GLC-82细胞增殖,阻断G0/G1期细胞周期。这种机制可能与P53蛋白的下调和Bcl-2蛋白表达的上调有关。关键词:肺肿瘤;腺癌;细胞增殖;细胞凋亡;左旋肉碱;表阿霉素
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of cell proliferation and apoptosis of L-carnitine combined with epirubicin on different human lung adenocarcinoma cells
Objective To investigate the effect of L-carnitine (LCNT) combined with epirubicin (EPI) on cell proliferation and apoptosis of two lung adenocarcinoma cell lines (GLC-82 and A549). Methods GLC-82 and A549 cells were divided into control group, EPI group, LCNT group and EPI+LCNT group, respectively. The cell proliferation rate was examined by MTT assay 24 h after the treatment, and the cell cycle and cell early apoptosis were analyzed by flow cytometry. The expression of P53 and Bcl-2 proteins were detected by Western Blot. Results Compared with the control group, the proliferation rate of GLC-82 and A549 cells was 37.56%(P 0.05) after 24 hours of 20.00 μg/ml LCNT treatment indicating LCNT could promote the proliferation of GLC-82 cells. Compared with the EPI group, EPI+LCNT had smaller inhibitory effect on the proliferation of GLC-82 cells, and the inhibition rate of the EPI+LCNT group was 12.14% lower than that of EPI group (P 0.05). Conclusions Comparing with the EPI, the combination of LCNT and EPI has less proliferation inhibition and apoptosis induction on GLC-82 cells, and without significant effect on A549 cells. LCNT can promote the proliferation of GLC-82 cells and block the cell cycle at G0/G1 phase. This mechanism may be related to down-regulation of P53 protein and up-regulation of Bcl-2 protein expression. Key words: Lung neoplasms; Adenocarcinoma; Cell proliferation; Cell apoptosis; L-carnitine; Epirubicin
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
1974
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信