热水法和超声法提取枳壳多糖的结构、链构象及抗氧化活性比较

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2020-01-01 DOI:10.1080/07328303.2020.1746329

Xiaomei Wang , Shaobo Zhang , Zhixu Li , Miao Wang , Runguang Sun

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引用次数: 5

摘要

采用红外光谱(IR)、气相色谱(GC)、气相色谱-质谱(GC- ms)、高铁酸盐氧化、Smith降解、核磁共振(NMR)、原子力显微镜(AFM)和刚果红(刚果红)等方法对热水和超声分离得到的两种枸橼酸多糖WPRL-DS和UPRL-DS的结构和链构象进行了研究。结果表明，超声提取法(UPRL-DS)的得率高于热水提取法(WPRL-DS)。upl - ds与WPRL-DS具有相同的一级结构。但upl - ds呈螺旋形，而WPRL-DS则不呈螺旋形。WPRL-DS和UPRL-DS均具有较强的抗氧化活性，包括羟基自由基、超氧阴离子和1,1-二苯基-2-苦基-肼基自由基清除能力。upl - ds比WPRL-DS表现出更强的抗氧化活性，且呈浓度依赖性。

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Comparison on structure, chain conformation, and antioxidant activity of polysaccharides extracted from Liriopes radix with hot water and ultrasound

The structures and chain conformations of two polysaccharides from Liriopes radix, WPRL-DS and UPRL-DS, isolated with hot water and ultrasound, respectively, were investigated by a combination of IR, GC, GC-MS, periodate oxidation, Smith degradation, NMR, AFM, and Congo red test. The results proved that the yield of ultrasonic extraction (UPRL-DS) was higher than that of hot water extraction (WPRL-DS). UPRL-DS had the same primary structure as WPRL-DS. However, UPRL-DS was spirally shaped whereas WPRL-DS was not. Both WPRL-DS and UPRL-DS were proved to have significant antioxidant activities as evidenced by their hydroxyl radical, superoxide anion, and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activities. UPRL-DS exhibited more effective antioxidant activity than WPRL-DS in a concentration-dependent manner.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.