血清学揭示加纳霍霍市恶性疟原虫传播的微小差异

Eric Kyei-Baafour, K. Kusi, Mavis Oppong, A. Frempong, Belinda Aculley, E. Ofori, M. Theisen, M. Kweku, B. Adu, L. Hviid, M. Ofori
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摘要

背景由于全球努力,疟疾传播率下降,需要一种更敏感的工具来监测微观层面的传播强度和模式。尽管在更广泛的地区传播可能相似,但社区内的卫生设施、露天蓄水、清晨和傍晚活动、户外睡眠和农业实践等因素可能会导致暴露差异,从而导致传播。因此,这项研究利用血清学在加纳霍霍市的微观层面上探讨了疟疾的传播。方法这项横断面研究涉及霍霍市农村(196)和城市(131)327名1-12岁的无症状儿童。使用间接ELISA在从干血点洗脱的血浆中测定对三种恶性疟原虫抗原(CSP、MSP2-FC27、MSP2-3D7)特异性的总IgG应答。结果显微镜和聚合酶链式反应检测结果显示,农村地区有较高比例的个体携带寄生虫。与城市社区相比,农村社区的总IgG水平和血清流行率较高(p<0.05)。在多元回归模型中,经混杂因素调整后,PfMSP2-3D7特异性IgG水平与农村社区的较高传播率有关。结论尽管该地区被归类为中度疟疾传播区,但定居点内的差异可能会影响疟疾传播,反映在抗体水平和疟疾抗原特异性IgG的流行率上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serology reveals micro-differences in Plasmodium falciparum transmission in the Hohoe municipality of Ghana
Background With the decline in malaria transmission due to global efforts, a more sensitive tool is needed to monitor transmission intensity and pattern at the micro-level. Though transmission in a broader area may be similar, factors such as sanitation, practices of open water storage, early morning and evening activities, outdoor sleeping and agricultural practices within communities could cause differences in exposure and thus transmission. This study thus probed malaria transmission at a micro-level using serology in the Hohoe Municipality of Ghana. Methods This cross-sectional study involved 327 asymptomatic children aged 1-12 years in both rural (196) and urban (131) communities in the Hohoe municipality. Total IgG responses specific for three P. falciparum antigens (CSP, MSP2-FC27, MSP2-3D7) were determined in plasma eluted from dried blood spots using indirect ELISA. Results A higher proportion of individuals in the rural area had parasites by both microscopy and PCR. Total IgG levels and seroprevalence were higher in rural compared to urban communities (p<0.05). In a multiple regression model, adjusting for confounders, levels of PfMSP2-3D7-specific IgG was associated with the higher transmission which occurs in the rural community. Conclusion The results suggest that though the district is categorized as having medium malaria transmission, differences within settlements may influence malaria transmission reflecting in antibody levels and prevalence of malaria antigen-specific IgG.
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