The effect of Saccharin on spermatogenes, testiscular tissue and pituitary –gonad hormones axis in rats

male Wistar classified into 5 equal groups in a completely randomized design. The control group did not receive any solvent or The control group received 1 ml distilled water per day as an intraperitoneal injection. Experimental groups received 1, 2 and 3 times daily, respectively, 250, 500 and 1000 mg / kg body weight saccharin intraperitoneal injection for 35 days, respectively. Blood samples were taken from animals after 35 days and testis tissue was isolated for histological examination. The obtained data were analyzed by SPSS software version 16 and one way ANOVA and Duncan's post hoc test. sperm in experimental groups have decreased significantly compared to the sham and control groups; but Sertoli and Leydig cells showed no any significant difference (P>0.05). Saccharin can have a harmful effects on the rate of hormone and cells, and the confirms the obtained hormonal results. Of course in this regard, the mode and dosage of using this common used artificial sweeter is very important.