水仙花晚季黄化病毒的新宿主木犀

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2021-11-15 DOI:10.36253/phyto-12709

J. Ágoston, A. Almási, K. Salánki, L. Palkovics

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引用次数: 1

摘要

2017年秋天，在匈牙利布达佩斯的一个植物园里，人们观察到了叶子上有黄绿色条纹的秋季水仙花植物。指示植物接种了疫苗，但症状没有出现。指示植物的RT-PCR测试也对病毒呈阴性。对有症状的木犀叶样品进行了Potyvirus特异性ACP-ELISA和RT-PCR。使用通用的potyvirus引物的RT-PCR产生一个大约1700个碱基对的PCR产物。外壳蛋白核苷酸序列的系统发育分析表明，其与日本分离的水仙花晚季黄病毒的同源性为98.78-99.51%。虽然之前已经报道了秋季水仙花的不明potyvirus感染，但序列数据尚未公布。因此，这是首次报道木犀作为水仙花晚季黄化病毒的宿主。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sternbergia lutea, a new host of Narcissus late season yellows virus

In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Indicator plants were inoculated, but symptoms did not develop. RT-PCR tests of the indicator plants were also negative for the viruses. Potyvirus specific ACP-ELISA and RT-PCR were carried out on the symptomatic S. lutea leaf samples. RT-PCR with universal potyvirus primers resulted in one, approx. 1700 base pair PCR product. Phylogenetic analysis of the nucleotide sequence of the coat protein demonstrated 98.78-99.51% identity with three Japanese isolates of Narcissus late season yellows virus. While unidentified potyvirus infection of autumn daffodil has been previously reported, sequence data have not been published. Therefore, this is the first report of Sternbergia lutea as a host of Narcissus late season yellows virus.

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.