天然深共晶溶剂存在下HMFO变体催化外消旋苯并稠醇的动力学拆分

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biocatalysis and Biotransformation Pub Date : 2022-02-11 DOI:10.1080/10242422.2022.2038582

G. de Gonzalo, Nikola Lončar, M. Fraaije

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引用次数: 1

摘要

摘要5-羟甲基糠醛氧化酶（HMFO）已被证明是一种有用的生物催化剂，用于使用氧作为温和氧化剂而不需要昂贵的有机辅因子的醇的选择性氧化。这种野生型HMFO生物催化剂和工程热稳定变体已经在不同苯并稠合醇的动力学拆分中进行了测试。天然深共晶溶剂在HMFO催化的醇氧化中的应用也进行了探索。在60%v/v的不同NADES存在下，外消旋1-茚醇的氧化显示出更高的转化率和选择性，特别是对于那些含有碳水化合物的NADES。通过适当选择生物催化剂和NADES，可以获得良好的对映选择性值，证明了在生物催化过程中使用这些新型溶剂的优点。

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Kinetic resolution of racemic benzofused alcohols catalysed by HMFO variants in presence of natural deep eutectic solvents

Abstract 5-Hydroxymethylfurfural oxidase (HMFO) has demonstrated to be a useful biocatalyst for the selective oxidation of alcohols employing oxygen as mild oxidant with no requirement of expensive organic cofactors. This wild-type HMFO biocatalyst and an engineered thermostable variant have been tested in the kinetic resolution of different benzofused alcohols. The use of natural deep eutectic solvents was also explored in HMFO-catalysed oxidation of alcohols. The oxidation of racemic 1-indanol showed a higher conversion and selectivity in presence of 60% v/v of different NADES, especially for those containing carbohydrates. By choosing properly the biocatalyst and the NADES, good enantioselectivity values can be obtained, demonstrating the advantages of employing these neoteric solvents in biocatalysed processes.

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来源期刊

Biocatalysis and Biotransformation 生物-生化与分子生物学

CiteScore

4.40

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： Biocatalysis and Biotransformation publishes high quality research on the application of biological catalysts for the synthesis, interconversion or degradation of chemical species. Papers are published in the areas of: Mechanistic principles Kinetics and thermodynamics of biocatalytic processes Chemical or genetic modification of biocatalysts Developments in biocatalyst''s immobilization Activity and stability of biocatalysts in non-aqueous and multi-phasic environments, including the design of large scale biocatalytic processes Biomimetic systems Environmental applications of biocatalysis Metabolic engineering Types of articles published are; full-length original research articles, reviews, short communications on the application of biotransformations, and preliminary reports of novel catalytic activities.