Laura L. López-Galindo, Ernesto Larios‐Soriano, Claudia Ventura-López, F. Díaz, D. Re, C. Galindo-Sánchez
{"title":"眼柄消融和驯化温度对凡纳滨对虾幼虾耐热性的影响:细胞保护氧化应激的分子生物标志物及其补偿机制","authors":"Laura L. López-Galindo, Ernesto Larios‐Soriano, Claudia Ventura-López, F. Díaz, D. Re, C. Galindo-Sánchez","doi":"10.3856/vol51-issue2-fulltext-2926","DOIUrl":null,"url":null,"abstract":"This study investigated the effect of acclimation temperatures and eyestalk ablation (EA) on the thermal tolerance of juvenile Penaeus vannamei. In each case, the relative expression of genes involved in cell protection (hsp70 and hsp90), oxidative stress (cMnSOD and GPx), and anaerobic metabolism (hif1a) was assessed. For this purpose, shrimp were acclimated to 20, 26, and 32°C for 21 days. After acclimation, the critical thermal maxima (CTmax) was determined in non-eyestalk ablated, unilaterally, and bilaterally eyestalk ablated organisms. An effect of acclimation temperatures on CTmax values was observed, with shrimp acclimated at 32°C having the highest rates. Likewise, EA resulted in lower thermal tolerance to CTmax in organisms acclimated at 20 and 26°C. The shrimp's protective and cellular repair responses were evidenced by increased hsp70 and hsp90 gene expression after CTmax and were intensified by the EA. In contrast, the results showed that cMnSOD was very sensitive to CTmax, and its expression was intensified with EA, while for GPx, there was an increase in the relative gene expression, mainly in shrimp acclimated at 20°C. In the case of hif1a, overexpression was observed at the acclimation temperature of 26°C, showing the activation of compensatory mechanisms such as anaerobic metabolism. EA caused a significant molecular response during CTmax of molecular biomarkers involved in heat stress response, oxidative stress, and compensatory mechanisms.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Eyestalk ablation and acclimation temperatures affect juveniles Penaeus vannamei thermo-tolerance: molecular biomarkers of cell protection oxidative stress, and compensatory mechanisms\",\"authors\":\"Laura L. López-Galindo, Ernesto Larios‐Soriano, Claudia Ventura-López, F. Díaz, D. Re, C. Galindo-Sánchez\",\"doi\":\"10.3856/vol51-issue2-fulltext-2926\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study investigated the effect of acclimation temperatures and eyestalk ablation (EA) on the thermal tolerance of juvenile Penaeus vannamei. In each case, the relative expression of genes involved in cell protection (hsp70 and hsp90), oxidative stress (cMnSOD and GPx), and anaerobic metabolism (hif1a) was assessed. For this purpose, shrimp were acclimated to 20, 26, and 32°C for 21 days. After acclimation, the critical thermal maxima (CTmax) was determined in non-eyestalk ablated, unilaterally, and bilaterally eyestalk ablated organisms. An effect of acclimation temperatures on CTmax values was observed, with shrimp acclimated at 32°C having the highest rates. Likewise, EA resulted in lower thermal tolerance to CTmax in organisms acclimated at 20 and 26°C. The shrimp's protective and cellular repair responses were evidenced by increased hsp70 and hsp90 gene expression after CTmax and were intensified by the EA. In contrast, the results showed that cMnSOD was very sensitive to CTmax, and its expression was intensified with EA, while for GPx, there was an increase in the relative gene expression, mainly in shrimp acclimated at 20°C. In the case of hif1a, overexpression was observed at the acclimation temperature of 26°C, showing the activation of compensatory mechanisms such as anaerobic metabolism. 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Eyestalk ablation and acclimation temperatures affect juveniles Penaeus vannamei thermo-tolerance: molecular biomarkers of cell protection oxidative stress, and compensatory mechanisms
This study investigated the effect of acclimation temperatures and eyestalk ablation (EA) on the thermal tolerance of juvenile Penaeus vannamei. In each case, the relative expression of genes involved in cell protection (hsp70 and hsp90), oxidative stress (cMnSOD and GPx), and anaerobic metabolism (hif1a) was assessed. For this purpose, shrimp were acclimated to 20, 26, and 32°C for 21 days. After acclimation, the critical thermal maxima (CTmax) was determined in non-eyestalk ablated, unilaterally, and bilaterally eyestalk ablated organisms. An effect of acclimation temperatures on CTmax values was observed, with shrimp acclimated at 32°C having the highest rates. Likewise, EA resulted in lower thermal tolerance to CTmax in organisms acclimated at 20 and 26°C. The shrimp's protective and cellular repair responses were evidenced by increased hsp70 and hsp90 gene expression after CTmax and were intensified by the EA. In contrast, the results showed that cMnSOD was very sensitive to CTmax, and its expression was intensified with EA, while for GPx, there was an increase in the relative gene expression, mainly in shrimp acclimated at 20°C. In the case of hif1a, overexpression was observed at the acclimation temperature of 26°C, showing the activation of compensatory mechanisms such as anaerobic metabolism. EA caused a significant molecular response during CTmax of molecular biomarkers involved in heat stress response, oxidative stress, and compensatory mechanisms.
期刊介绍:
Latin American Journal of Aquatic Research- LAJAR is the continuation of the journal Investigaciones Marinas (1970-2007) and is published since 2008 by the Escuela de Ciencias del Mar, Facultad de Ciencias del Mar y Geografía of the Pontificia Universidad Católica de Valparaíso. LAJAR is an “Open Access” journal that publishes in English language, original research articles, reviews and short communications on aquatic science, which contain the results of research conducted in aquaculture or in oceanic and coastal marine waters of Latin America.
The following topics are considered: Physical Oceanography, Chemical Oceanography, Marine Biogeochemistry, Marine Pollution and Toxicology, Marine Geology and Geophysics, Biological Oceanography, Fisheries and Aquaculture.