Evaluation of Salivary 8-Isoprostane in Oral Lichen Planus: Case Control Study

Introduction: Lichen planus is a chronic inflammatory disease that affects the skin, nails, and the mucous membrane and oral mucosal involvement may frequently precedes skin manifestation. It is hypothesized that free radicals and reactive oxygen species has a strong relationship in the pathogenesis of oral lichen planus. F2-isoprostane are a group of bioactive prostaglandins and they are isomers of PGF2α is a most accurate approach to assess oxidant injury in vivo. The aim of the study was to evaluate the level of 8-isoprostane in saliva of patients with erosive and nonerosive oral lichen planus in comparison with healthy controls. Materials and Methods: The study participants were selected by convenience sampling from department of oral medicine and dermatology department of our institution. In this case control study, 36 patients with oral lichen planus and 36 control subjects were included. The lichen planus group is again subdivided into erosive and nonerosive lichen planus group. Unstimulated saliva was collected by passive drool method. The salivary 8–isoprostane level was measured by using 8-isoprostane ELISA kit. The statistical tests used were Chi square test, ANOVA and Pearson correlation. Results: The mean values of the salivary 8-isoprostane level an increase in the oral lichen planus group when compared with control group and is statistically significant (P ˂ 0.05). Conclusion: The present study showed that oxidative stress was increased in patients with oral lichen planus, reflected by higher concentration of salivary 8 isoprostane being higher in erosive lichen planus than nonerosive lichen planus.