{"title":"短叶苜蓿体细胞胚胎发生过程中NF-Y新基因的表达","authors":"Elina Potsenkovskaia , Varvara Tvorogova , Daria Yakovleva , Natalia Zlydneva , Ludmila Lutova","doi":"10.1016/j.plgene.2022.100364","DOIUrl":null,"url":null,"abstract":"<div><p><span>Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and </span><em>in vitro</em> propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the <em>NF-Y</em> family and to analyse the mechanism of their action in <span><em>Medicago</em><em> truncatula</em></span>. We found that two LEC1-type <em>NF-YB</em> genes, <em>MtNF-YB3</em> and <em>10</em>, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in <em>M. truncatula</em>. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from <em>NF-YA</em> and <em>C</em> groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, <em>MtNF-YA3</em>, increased callus weight. The results obtained provide new information about <em>NF-Y</em><span> genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.</span></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula\",\"authors\":\"Elina Potsenkovskaia , Varvara Tvorogova , Daria Yakovleva , Natalia Zlydneva , Ludmila Lutova\",\"doi\":\"10.1016/j.plgene.2022.100364\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and </span><em>in vitro</em> propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the <em>NF-Y</em> family and to analyse the mechanism of their action in <span><em>Medicago</em><em> truncatula</em></span>. We found that two LEC1-type <em>NF-YB</em> genes, <em>MtNF-YB3</em> and <em>10</em>, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in <em>M. truncatula</em>. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from <em>NF-YA</em> and <em>C</em> groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, <em>MtNF-YA3</em>, increased callus weight. The results obtained provide new information about <em>NF-Y</em><span> genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.</span></p></div>\",\"PeriodicalId\":38041,\"journal\":{\"name\":\"Plant Gene\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352407322000142\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352407322000142","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula
Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type NF-YB genes, MtNF-YB3 and 10, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in M. truncatula. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from NF-YA and C groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, MtNF-YA3, increased callus weight. The results obtained provide new information about NF-Y genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.
Plant GeneAgricultural and Biological Sciences-Plant Science
CiteScore
4.50
自引率
0.00%
发文量
42
审稿时长
51 days
期刊介绍:
Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.