Guo Feng, Wenjing Wang, Lai-lai Li, Wei Li, Wen Liu, Zeng-guang Wu, Hongmei Su, G. Zhu, Chenchen Ren, Xueli Song, Ju Zhang, Zhengyan He
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Daphnoretin and IS (buspirone) were chromatographed on an agilent Zorbax XDB-C18 column (2.1 mm × 50 mm, 3.5 μm), and Gradient elution of acetonitrile-0.15% formic acid in aqueous solution.\nQuantification was performed using electrospray ionization in positive ion multiple reaction monitoring mode of the transitions m/z 353.1→179.1 for daphnoretin and m/z 386.3→122.3 for IS.\n\n\n\nGood linearity between 5–10000 ng/mL for cyperidin in plasma and tissue samples (r ≥ 0.99) was resulted. The accuracies of plasma and tissue homogenates ranged from –3.31% to 9.00%, and the precision was less than 5.78%. After that, the validated method was successfully applied to the pharmacokinetics and tissue distribution study of daphnoretin after oral administration of ethanol extract from the roots of RWI to rats.\n\n\n\nDaphnoretin was well absorbed in the systemic circulation after oral administration and was widely distributed in tissues, with the highest concentration in lung tissue. This study is beneficial to the development and utilization of RWI and provides a reasonable reference for its clinical administration.\n\n\n\nnone\n","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pharmacokinetics and Tissue Distribution Study of Daphnoretin in Ethanol Extract from the Roots of Wikstroemia Indica in Rats by a Validated UPLC-MS/MS Method\",\"authors\":\"Guo Feng, Wenjing Wang, Lai-lai Li, Wei Li, Wen Liu, Zeng-guang Wu, Hongmei Su, G. Zhu, Chenchen Ren, Xueli Song, Ju Zhang, Zhengyan He\",\"doi\":\"10.2174/1573412919666230223140457\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n\\nDaphnoretin, as a known bicoumarin compound that contained various pharmacological activities, was isolated from Wikstroemia indica C.A. 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引用次数: 0
摘要
从紫薇属植物(Wikstroemia indica c.a.mey, RWI)中分离得到了一种具有多种药理活性的双香豆素类化合物。本研究旨在研究瑞香乙醇提取物中瑞香素在大鼠血浆中的药动学特征,并建立快速、可靠、灵敏的超高效液相色谱串联质谱法测定大鼠血浆及各组织中瑞香素的含量。建立了UPLC-MS/MS方法。采用agilent Zorbax XDB-C18色谱柱(2.1 mm × 50 mm, 3.5 μm)对丹参素和丁螺环酮进行层析,乙腈-0.15%甲酸水溶液梯度洗脱。采用电喷雾电离法在正离子多反应监测模式下对m/z 353.1→179.1和m/z 386.3→122.3过渡段进行定量。血浆和组织样品中cyperidin含量在5 ~ 10000 ng/mL之间呈良好的线性关系(r≥0.99)。血浆和组织匀浆的准确度为-3.31% ~ 9.00%,精密度小于5.78%。之后,将验证的方法成功应用于大鼠口服大黄根乙醇提取物后大黄皮苷的药代动力学和组织分布研究。口服给药后,瑞香素在体循环中吸收良好,在组织中分布广泛,以肺组织中浓度最高。本研究有利于RWI的开发利用,为其临床应用提供合理参考
Pharmacokinetics and Tissue Distribution Study of Daphnoretin in Ethanol Extract from the Roots of Wikstroemia Indica in Rats by a Validated UPLC-MS/MS Method
Daphnoretin, as a known bicoumarin compound that contained various pharmacological activities, was isolated from Wikstroemia indica C.A. Mey (RWI).
The study aims to investigate the pharmacokinetic characteristics of daphnoretin from RWI ethanol extracts in rat plasma and to determine daphnetin in rat plasma and various tissues by a rapid, reliable and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method.
The UPLC-MS/MS method was established. Daphnoretin and IS (buspirone) were chromatographed on an agilent Zorbax XDB-C18 column (2.1 mm × 50 mm, 3.5 μm), and Gradient elution of acetonitrile-0.15% formic acid in aqueous solution.
Quantification was performed using electrospray ionization in positive ion multiple reaction monitoring mode of the transitions m/z 353.1→179.1 for daphnoretin and m/z 386.3→122.3 for IS.
Good linearity between 5–10000 ng/mL for cyperidin in plasma and tissue samples (r ≥ 0.99) was resulted. The accuracies of plasma and tissue homogenates ranged from –3.31% to 9.00%, and the precision was less than 5.78%. After that, the validated method was successfully applied to the pharmacokinetics and tissue distribution study of daphnoretin after oral administration of ethanol extract from the roots of RWI to rats.
Daphnoretin was well absorbed in the systemic circulation after oral administration and was widely distributed in tissues, with the highest concentration in lung tissue. This study is beneficial to the development and utilization of RWI and provides a reasonable reference for its clinical administration.
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期刊介绍:
Aims & Scope
Current Pharmaceutical Analysis publishes expert reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.