不同溶剂对长椒果实提取物提取、生药学评价及抗氧化活性的影响

A. Mittal
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引用次数: 0

摘要

研究了不同溶剂对辣椒果实提取物提取、生药学评价和抗氧化活性的影响。采用水、乙醇、甲醇、丙酮和乙酸乙酯溶解法提取了长叶果粉末中的生物分析物。此外,提取液在55°C下使用旋蒸干燥,并用于评估植物化学分析,其中使用Folin-Ciocalteu Assay评估总酚化合物(TPCs)。此外,测定了抗坏血酸的含量,并进行傅里叶变换红外光谱(FTIR)分析,根据其表面官能团预测各种化合物的存在。此外,通过考虑DPPH自由基清除活性来确定抗氧化效率。TPC和抗坏血酸的定量测定结果表明,龙骨中存在生药学成分,FTIR分析也证实了这一结论。乙酸乙酯部位多酚含量最高(181.0±8.69 mg AE/g),甲醇部位抗坏血酸含量最高(1.51±0.067 mg AE/g)。甲醇和乙酸乙酯萃取法对DPPH自由基的清除率最高,达到81.57%,其次是乙酸乙酯(79.35%)。研究结果表明,龙葵提取物可作为制药工业和地方一级的原料,以改善卫生条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential Effects of Solvents on Extraction, Pharmacognostic Evaluation and Antioxidant Activity of Long Pepper Piper longum Fruit Extract
The present study proposed differential effect of solvents on extraction, pharmacognostic evaluation and antioxidant activity of Long pepper (Piper longum) fruit extract. The bio-analytes present in P. longum fruit were extracted by dissolving powder fruit in water, ethanol, methanol, acetone, and ethyl acetate. Further, the extracts were dried using rotavapor at 55 °C and used to assess the phytochemical analysis where total phenolic compounds (TPCs) were evaluated using Folin-Ciocalteu Assay. Additionally, ascorbic acid content was measured, and Fourier Transform Infrared Spectroscopy (FTIR) analysis was performed to predict the presence of various compounds based on their surface functional groups. Further, antioxidant efficiency was determined by considering DPPH radical scavenging activity. The quantitative measurements of the TPC and ascorbic acid reveal the presence of the pharmacognostic compounds in P. longum, which is also supported by FTIR analysis. The FC assay test confirmed the highest polyphenols (181.0 ± 8.69 mg GAE/g) in ethyl acetate extract, while methanolic extract showed the maximum ascorbic acid content (1.51 ± 0.067 mg AE/g). Methanolic and ethyl acetate-based extraction process showed great results than other solvents as they showed maximum DPPH radical scavenging activity (% degradation), 81.57 %, followed by ethyl acetate (79.35%). The obtained results announced P. longum extract can be used as a raw material in pharmaceutical industries and local levels to improve health conditions.
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来源期刊
Bioscience Biotechnology Research Communications
Bioscience Biotechnology Research Communications BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
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