活细胞片上低温保存的研究进展

Biao Wang
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引用次数: 0

摘要

随着分子、细胞和微组织、器官模型逐渐融入微流控芯片,微流控芯片的平台价值在生物医学领域日益凸显。开发芯片上细胞样品的冷冻保存不仅是实现这一价值的必要条件,也是对传统细胞样品冷冻保存的有益创新。本文综述了细胞样品在微流控芯片上的冷冻保存技术,包括细胞样品在芯片上的缓慢冷冻和冷冻过程中的温度控制,细胞样品玻璃化的理论分析和验证。此外,还介绍了在非低温下在芯片上保存细胞样品的方法。最后,简要分析了细胞样品芯片冷冻保存的动力学和存在的问题,为相关研究提供参考。关键词:低温保存;微流控芯片;细胞样本
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Research progress in on-chip cryopreservation of living cells
With the gradual integration of molecules, cells and micro tissues, organ models into microfluidic chips, the platform value of microfluidic chips is increasingly prominent in biomedical field. The development of cryopreservation of cell samples on chip is not only the essential condition for realizing this value, but a beneficial innovation for traditional cryopreservation of cell samples. In this paper, the cryopreservation techniques of cell samples on microfluidic chips were reviewed, including slow freezing of cell samples on chips and temperature control in this process, theoretical analysis and verification of vitrification of cell samples on chips. Furthermore, preservation of cell samples on chips at non-cryogenic temperature was introduced. Finally, the dynamics and problems in on-chip cryopreservation of cell samples were briefly analyzed to provide reference for relative researches. Key words: Cryopreservation; Microfluidic chips; Cell samples
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