UHPLC-ESI-Q-TOF/MS研究马齿苋酮B在大鼠体内的代谢

IF 0.7 4区医学 Q4 PHARMACOLOGY & PHARMACY

Current Pharmaceutical Analysis Pub Date : 2023-03-06 DOI:10.2174/1573412919666230306124751

Aijing Leng, Xixiang Ying, Xinyu Cui, Xiujuan Lan

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引用次数: 0

摘要

目的：研究马齿苋科生物碱马齿苋酮B在大鼠体内的主要代谢产物和代谢途径。研究表明，马齿苋酮B具有良好的抗炎和抗胆碱酯酶活性，这一点有待进一步研究。Portulacatone B通过大鼠的尾静脉给药，在10分钟和30分钟时收集眼眶血，并在24小时内收集尿液和粪便。采用超高效液相色谱-电喷雾-四极杆飞行时间质谱联用技术（UHPLC-ESI-Q-TOF/MS）研究了马齿苋酮B在大鼠体内的主要代谢产物和代谢途径，从马齿苋中分离得到的生物碱。对马齿苋酮B的代谢产物和代谢途径的研究结果表明，经大鼠尾静脉给药后，在血浆样品中发现3种代谢产物，在尿液样品中发现2种代谢产物和在粪便样品中发现1种代谢产物。主要代谢途径是氧化、水解、甲基化、葡萄糖醛酸化和磺化。Portulacatone B通过大鼠的尾静脉给药，收集10分钟和30分钟的眼眶血以及24小时内的尿液和粪便。采用超高效液相色谱-电喷雾-四极杆飞行时间质谱联用技术（UHPLC-ESI-Q-TOF/MS）对大鼠体内代谢产物和代谢途径进行了研究，在大鼠血浆、尿液和粪便样品中发现6种代谢产物，代谢途径包括氧化、水解、甲基化、葡萄糖醛酸化和磺化过程。对马齿苋酮B的代谢产物和代谢途径的研究结果表明，通过大鼠尾静脉给药后，在血浆样品中发现3种代谢产物；尿液样品中的2种代谢产物和粪便样品中的一种代谢产物。主要的代谢途径是氧化、水解、甲基化、葡萄糖醛酸化和磺化

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The Metabolism of Portulacatone B from Portulaca oleracea L. in rats by UHPLC-ESI-Q-TOF/MS

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The Metabolism of Portulacatone B from Portulaca oleracea L. in rats by UHPLC-ESI-Q-TOF/MS

Objective: This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which is an alkaloid isolated from Portulaca oleracea L. Portulaca oleracea L. is an edible and medicinal plant belonging to the family of Portulacaceae, with rich pharmacological effects mainly attributed to its active ingredients. Studies have shown that Portulacatone B has excellent anti-inflammatory and anticholinesterase activity, which was further investigated. Portulacatone B was administered through the tail vein of the rat, and the orbital blood at 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathways in the rat were researched by ultra-high performance liquid chromatography-electrospray coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS). This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which was an alkaloid isolated from Portulaca oleracea L. The research results of the metabolites and metabolic pathways of Portulacatone B showed that after administration through the tail vein of rats, 3 metabolites were found in the plasma sample, 2 metabolites in the urine sample, and one metabolite in the feces sample. The main metabolic pathways were found to be oxidation, hydrolysis, methylation, glucuronidation, and sulfonation. Portulacatone B was administered through the tail vein of the rat, the orbital blood of 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathway in the rat were researched by ultra-high performance liquid chromatography electrospray coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS). Six metabolites were found in the rat’s plasma, urine, and feces samples, and the metabolic pathways included oxidation, hydrolysis, methylation, glucuronidation, and sulfonation process. The research results of the metabolites and metabolic pathways of Portulacatone B showed that after administration through the tail vein of rats, 3 metabolites were found in the plasma sample; 2 metabolites in the urine sample, and a metabolite in the feces sample. The main metabolic pathways are oxidation, hydrolysis, methylation, glucuronidation, and sulfonation. not

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来源期刊

Current Pharmaceutical Analysis 医学-药学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Aims & Scope Current Pharmaceutical Analysis publishes expert reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.