美洲Persea americana种子水提取物对α-葡萄糖苷酶抑制剂的筛选

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
T. A. Lawal
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引用次数: 0

摘要

胃肠道中α-葡萄糖苷酶的活性与餐后高血糖有关。如果控制不当,餐后高血糖可能发展为糖尿病,一种代谢综合征。糖尿病与许多并发症有关,如视网膜病变、心脏病发作、肾病、神经病变、中风和下肢截肢。目前使用的抗糖尿病药物对餐后血糖漂移几乎没有影响,因此不会将血糖水平降至基线。本研究提取、分级并筛选了美洲Persea americana种子的水提取物的低血糖潜力。研究了提取物的级分和亚级分对α-葡萄糖苷酶活性的抑制作用。对最具活性的亚组分进行傅立叶变换红外光谱(FTIR)和气相色谱-质谱(GC-MS)分析,以阐明活性成分。活性亚组分对α-葡萄糖苷酶有显著的抑制作用(p<0.05)。该亚组分竞争性抑制α-葡萄糖苷酶(IC50 = 9.48 ± 0.58 μg/mL),尽管药效低于标准药物阿卡波糖(IC50 = 6.45 ± 0.47 μg/mL)。亚组分的FTIR分析显示存在羰基、羟基、羧基、双键、亚甲基和甲基。GC-MS分析表明存在顺式-11,14-二十碳二烯酸、儿茶素和绿原酸作为活性成分。总之,从这项研究中获得的成分可以在实验室中合成,以进一步证实其降血糖活性。可以进一步探索最活性的亚组分,以确认其对酶的抑制活性,并确定其在体内治疗糖尿病中的程度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of Aqueous Extract of Persea americana Seeds for Alpha-Glucosidase Inhibitors
Activity of α-glucosidase enzyme in the gastrointestinal tract has been implicated in postprandial hyperglycaemia. If not properly controlled, postprandial hyperglycaemia might progress to diabetes mellitus, a metabolic syndrome. Diabetes is associated with many complications such as retinopathy, heart attack, nephropathy, neuropathy, stroke, and lower limb amputation. Antidiabetic medications presently in use have little effect on postprandial glycaemic excursion and hence do not bring down the blood glucose level to baseline. This study extracted, fractionated, and screened the aqueous extract of Persea americana seeds for hypoglycaemic potential. Inhibitory effects of the fractions and subfractions of the extract on α-glucosidase activity were investigated. The most active subfraction was subjected to Fourier transform infrared (FTIR) and gas chromatography mass spectroscopy (GC-MS) analysis to elucidate the active components. The active subfraction showed a significant inhibition (p < 0.05) on α-glucosidase. The subfraction competitively inhibits α-glucosidase (with IC50 = 09.48 ± 0.58 μg/mL), though less potent than the standard drug, acarbose (IC50 = 06.45 ± 0.47 μg/mL). FTIR analysis of the subfraction showed the presence of carbonyl group, hydroxy group, carboxyl group, double bonds, methylene, and methyl groups. GC-MS analysis suggests the presence of cis-11,14-eicosadienoic acid, catechin, and chlorogenic acid as the active components. In conclusion, the components obtained from this study can be synthesised in the laboratory to further confirm their hypoglycaemic activity. The most active subfraction can be explored further to confirm its inhibitory activity against the enzyme and to determine its extent in the treatment of diabetes mellitus in vivo.
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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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