抗菌丝胶/聚乙烯醇水凝胶具有抗真菌性能,用于潜在感染的大面积烧伤创面愈合:系统评价

Q1 Engineering
Bianza Moise Bakadia , Lallepak Lamboni , Abeer Ahmed Qaed Ahmed , Ruizhu Zheng , Biaou Oscar Ode Boni , Zhijun Shi , Shuyao Song , Tiatou Souho , Biampata Mutu Mukole , Fuyu Qi , Guang Yang
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引用次数: 18

摘要

水凝胶型烧伤创面敷料具有良好的抗菌、抗真菌和力学性能,是促进大面积感染烧伤创面愈合的理想生物材料。本研究以吉尼平(GNP)为交联剂,采用重复冻融法制备了聚乙烯醇(PVA)、丝胶蛋白(SS)和阿奇霉素(AZM)为主要原料的水凝胶。FTIR结果表明,所有水凝胶组分均成功混合。SS掺入提高了水凝胶的溶胀率、孔隙度、细胞附着和增殖,PVA含量的增加提高了水凝胶的力学性能。AZM的加入提高了水凝胶对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌和白色念珠菌的抗菌性能。水凝胶显示出持续的SS和AZM释放以及对角质形成细胞和成纤维细胞的细胞相容性。此外,水凝胶在冷冻干燥时表现出皮肤粘附能力。在体内研究中,使用感染的小鼠全层烧伤模型,其体表面积为10%,结果表明,烧伤损伤导致炎症细胞因子反应增加,脾脏和肝脏的宏观和微观改变。另一方面,肾脏没有显示出任何变化。有趣的是,制备的水凝胶比商业Tegaderm™膜敷料具有更好的烧伤创面愈合效果,最大限度地减少了全身烧伤影响。因此,这种新型水凝胶有望成为加速感染烧伤创面愈合的有希望的候选物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antibacterial silk sericin/poly (vinyl alcohol) hydrogel with antifungal property for potential infected large burn wound healing: Systemic evaluation

Antibacterial silk sericin/poly (vinyl alcohol) hydrogel with antifungal property for potential infected large burn wound healing: Systemic evaluation

Hydrogel-based burn wound dressings with excellent antibacterial, antifungal, and mechanical properties are ideal biomaterials to promote infected large burn wound healing. In this study, the hydrogel synthesized by repetitive freezing-thawing consists of poly (vinyl alcohol) (PVA), silk sericin (SS), and azithromycin (AZM), with genipin (GNP) as crosslinker. The FTIR showed that all hydrogel components were successfully blended. The swelling ratio, porosity, cell attachment, and proliferation improved with SS incorporation, while increased PVA content enhanced the mechanical performance of the hydrogel. The inclusion of AZM improved the antimicrobial property of the hydrogel towards Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. The hydrogel showed sustained SS and AZM releases as well as cytocompatibility on keratinocytes and fibroblasts. Furthermore, the hydrogel displays skin adhesion ability when freeze-dried. In the in vivo study using an infected mouse full-thickness burn model with a 10% total body surface area, it was shown that burn injury led to increased inflammatory cytokine responses and macroscopic and microscopic alterations in the spleen and liver. The kidneys, on the other hand, revealed neither change. Interestingly, the prepared hydrogel had a better burn wound healing effect than the commercial Tegaderm™ film dressing, minimizing systemic burn effects. Hence, this novel hydrogel is projected to be a promising candidate for accelerated healing of infected burn wounds.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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