pmHAS酶的结构分析和工程设计以提高其功能性能:一项硅片研究

IF 1.2 4区化学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI:10.1080/07328303.2020.1821041

Alireza Zakeri , Sepideh Khoshsorour , Mohsen Karami Fath , Navid Pourzardosht , Faezeh Fazeli , Saeed Khalili

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引用次数: 1

摘要

据报道，多杀性巴氏杆菌透明质酸合成酶(pmHAS)能够解决透明质酸(HA)的多分散性问题，同时简化其纯化过程。在本研究中，我们试图设计一种具有改进功能特性的突变型pmHAS酶。在这方面，几个突变被预测并施加在酶的活性位点。结果表明，突变酶更稳定，能够以更高的亲和力与配体结合。根据我们的研究结果，这种突变酶可以更有效地产生透明质酸，并防止透明质酸的分解。

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Structural analyses and engineering of the pmHAS enzyme to improve its functional performance: An in silico study

The Pasteurella multocida hyaluronic acid synthase (pmHAS) is reported to be able to solve the problem of hyaluronic acid (HA) polydispersity, while simplifying its purification process. In the present study, we tried to design a mutant pmHAS enzyme with improved functional properties. In this regard, several mutations were predicted and exerted within the active site of the enzyme. The obtained results showed that the mutant enzyme was more stable and was able to bind to its ligands with higher affinity. Given our results, the mutated enzyme could be used to produce HA more efficiently and prevent the breakdown of HA.

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来源期刊

Journal of Carbohydrate Chemistry 化学-生化与分子生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Carbohydrate Chemistry serves as an international forum for research advances involving the chemistry and biology of carbohydrates. The following aspects are considered to fall within the scope of this journal: -novel synthetic methods involving carbohydrates, oligosaccharides, and glycoconjugates- the use of chemical methods to address aspects of glycobiology- spectroscopic and crystallographic structure studies of carbohydrates- computational and molecular modeling studies- physicochemical studies involving carbohydrates and the chemistry and biochemistry of carbohydrate polymers.