{"title":"核桃酮(5-羟基-1,4-萘醌)对氟康唑耐药和敏感念珠菌的体外活性研究","authors":"Afsane Vaezi , Masoud Moghadaszadeh , Elahe Nasri , Shima Gharibi , Kambiz Diba , Adam Matkowski , Hamed Fakhim","doi":"10.1016/j.riam.2022.01.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.</p></div><div><h3>Aims</h3><p>In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant <em>Candida</em> infections, we studied the <em>in vitro</em> susceptibility of juglone against fluconazole-susceptible and -resistance <em>Candida</em> isolates, alone and in combination.</p></div><div><h3>Methods</h3><p>Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.</p></div><div><h3>Results</h3><p>Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC<sub>50</sub> and MIC<sub>90</sub> of 0.031 and 0.5<!--> <!-->μg/mL. There were significant differences in MICs of fluconazole (<em>p</em> <!--><<!--> <!-->0.001) and juglone (<em>p</em> <!--><<!--> <!-->0.0003) between <em>Candida</em> <em>albicans</em> and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant <em>Candida</em> isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested <em>Candida</em> species.</p></div><div><h3>Conclusions</h3><p>Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates\",\"authors\":\"Afsane Vaezi , Masoud Moghadaszadeh , Elahe Nasri , Shima Gharibi , Kambiz Diba , Adam Matkowski , Hamed Fakhim\",\"doi\":\"10.1016/j.riam.2022.01.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.</p></div><div><h3>Aims</h3><p>In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant <em>Candida</em> infections, we studied the <em>in vitro</em> susceptibility of juglone against fluconazole-susceptible and -resistance <em>Candida</em> isolates, alone and in combination.</p></div><div><h3>Methods</h3><p>Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.</p></div><div><h3>Results</h3><p>Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC<sub>50</sub> and MIC<sub>90</sub> of 0.031 and 0.5<!--> <!-->μg/mL. There were significant differences in MICs of fluconazole (<em>p</em> <!--><<!--> <!-->0.001) and juglone (<em>p</em> <!--><<!--> <!-->0.0003) between <em>Candida</em> <em>albicans</em> and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant <em>Candida</em> isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested <em>Candida</em> species.</p></div><div><h3>Conclusions</h3><p>Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.</p></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2022-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1130140622000067\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140622000067","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates
Background
The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.
Aims
In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant Candida infections, we studied the in vitro susceptibility of juglone against fluconazole-susceptible and -resistance Candida isolates, alone and in combination.
Methods
Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.
Results
Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC50 and MIC90 of 0.031 and 0.5 μg/mL. There were significant differences in MICs of fluconazole (p < 0.001) and juglone (p < 0.0003) between Candidaalbicans and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant Candida isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested Candida species.
Conclusions
Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.
期刊介绍：
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
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