烟叶半边莲不同叶、根提取物自由基清除活性评价及EC50值估算(野生烟草)

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-06-14 DOI:10.1080/10496475.2021.1932006

R. Kolap, Prachi S. Kakade, R. Gacche, S. Zimare

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引用次数: 3

摘要

烟叶半边莲(Lobelia nicotianifolia Roth)叶和根溶剂提取物的抗氧化能力(% RSA和EC50)。采用五种体外测定法进行评估。甲醇提取物对一氧化氮(30.67µg mL−1)和过氧化氢(81.01µg mL−1)自由基的EC50值与抗坏血酸相当。% RSA和EC50与总酚含量和总黄酮含量相关。经液相色谱-高分辨率质谱分析鉴定，其主要成分为5种黄酮类化合物、6种酚类化合物、3种类胡萝卜素、1种蒽醌、香豆素、羟基醌和异黄酮。高效液相色谱法测定血凝素、没食子酸、槲皮素含量。

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Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco)

ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.