{"title":"日本橙蝇tsuneonbactrocera微卫星标记的研制(双翅目:绢蝇科)","authors":"Pattara Opadith, Satsuki Iwamoto, Minoru Narahara, Yoshio Okazaki, Yoshimitsu Higashiura, Jun Otake, Hajime Ono, Norihide Hinomoto","doi":"10.1007/s13355-022-00783-9","DOIUrl":null,"url":null,"abstract":"<div><p>To monitor and control the Japanese orange fly <i>Bactrocera tsuneonis</i> (Miyake) (Diptera: Tephritidae), it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for <i>B. tsuneonis.</i> A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0 and 0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations. A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of <i>B. tsuneonis</i>.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)\",\"authors\":\"Pattara Opadith, Satsuki Iwamoto, Minoru Narahara, Yoshio Okazaki, Yoshimitsu Higashiura, Jun Otake, Hajime Ono, Norihide Hinomoto\",\"doi\":\"10.1007/s13355-022-00783-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>To monitor and control the Japanese orange fly <i>Bactrocera tsuneonis</i> (Miyake) (Diptera: Tephritidae), it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for <i>B. tsuneonis.</i> A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0 and 0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations. A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of <i>B. tsuneonis</i>.</p></div>\",\"PeriodicalId\":8551,\"journal\":{\"name\":\"Applied Entomology and Zoology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Entomology and Zoology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13355-022-00783-9\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Entomology and Zoology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13355-022-00783-9","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)
To monitor and control the Japanese orange fly Bactrocera tsuneonis (Miyake) (Diptera: Tephritidae), it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for B. tsuneonis. A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0 and 0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations. A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of B. tsuneonis.
期刊介绍:
Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.