{"title":"应用piggyBac系统切除菊花DNA片段","authors":"Mitsuko Kishi-Kaboshi, Ayako Nishizawa-Yokoi, Ichiro Mitsuhara, Seiichi Toki, Katsutomo Sasaki","doi":"10.5511/plantbiotechnology.23.0324a","DOIUrl":null,"url":null,"abstract":"<p><p><i>Chrysanthemum morifolium</i> is one of the most popular ornamental plants in the world. However, as <i>C. morifolium</i> is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its trait systematically. Genome editing technology is one of the attractive methods for modifying traits systematically. For the commercial use of genetically modified <i>C. morifolium</i>, rigorous stabilization of its quality is essential. This trait stability can be achieved by avoiding further genome modification after suitable trait modification by genome editing. Since <i>C. morifolium</i> is a vegetatively propagated plant, an approach for removing genome editing tools is required. In this study, we attempted to use the <i>piggyBac</i> transposon system to remove specific DNA sequences from the <i>C. morifolium</i> genome. Using the luminescence as a visible marker, we demonstrated that inoculation of <i>Agrobacterium</i> harboring hyperactive <i>piggyBac</i> transposase removes inserted 2.6 kb DNA, which harbors <i>piggyBac</i> recognition sequences, from the modified Eluc sequence.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797517/pdf/","citationCount":"0","resultStr":"{\"title\":\"Excision of DNA fragments with the <i>piggyBac</i> system in <i>Chrysanthemum morifolium</i>.\",\"authors\":\"Mitsuko Kishi-Kaboshi, Ayako Nishizawa-Yokoi, Ichiro Mitsuhara, Seiichi Toki, Katsutomo Sasaki\",\"doi\":\"10.5511/plantbiotechnology.23.0324a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Chrysanthemum morifolium</i> is one of the most popular ornamental plants in the world. However, as <i>C. morifolium</i> is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its trait systematically. Genome editing technology is one of the attractive methods for modifying traits systematically. For the commercial use of genetically modified <i>C. morifolium</i>, rigorous stabilization of its quality is essential. This trait stability can be achieved by avoiding further genome modification after suitable trait modification by genome editing. Since <i>C. morifolium</i> is a vegetatively propagated plant, an approach for removing genome editing tools is required. In this study, we attempted to use the <i>piggyBac</i> transposon system to remove specific DNA sequences from the <i>C. morifolium</i> genome. Using the luminescence as a visible marker, we demonstrated that inoculation of <i>Agrobacterium</i> harboring hyperactive <i>piggyBac</i> transposase removes inserted 2.6 kb DNA, which harbors <i>piggyBac</i> recognition sequences, from the modified Eluc sequence.</p>\",\"PeriodicalId\":20411,\"journal\":{\"name\":\"Plant Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797517/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.5511/plantbiotechnology.23.0324a\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.5511/plantbiotechnology.23.0324a","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
菊花(Chrysanthemum morifolium)是世界上最受欢迎的观赏植物之一。然而,由于菊花是分段六倍体、自交不亲和、异源基因组庞大,因此很难对其性状进行系统改造。基因组编辑技术是有吸引力的系统改造性状的方法之一。要想将转基因大花蕙兰用于商业用途,就必须严格稳定其品质。通过基因组编辑技术对性状进行适当改造后,避免进一步的基因组改造,可以实现性状的稳定性。由于 C. morifolium 是一种无性繁殖植物,因此需要一种移除基因组编辑工具的方法。在这项研究中,我们尝试使用 piggyBac 转座子系统移除 C. morifolium 基因组中的特定 DNA 序列。利用发光作为可见标记,我们证明了接种携带超活性 piggyBac 转座酶的农杆菌可从修改后的 Eluc 序列中移除插入的 2.6 kb DNA,其中包含 piggyBac 识别序列。
Excision of DNA fragments with the piggyBac system in Chrysanthemum morifolium.
Chrysanthemum morifolium is one of the most popular ornamental plants in the world. However, as C. morifolium is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its trait systematically. Genome editing technology is one of the attractive methods for modifying traits systematically. For the commercial use of genetically modified C. morifolium, rigorous stabilization of its quality is essential. This trait stability can be achieved by avoiding further genome modification after suitable trait modification by genome editing. Since C. morifolium is a vegetatively propagated plant, an approach for removing genome editing tools is required. In this study, we attempted to use the piggyBac transposon system to remove specific DNA sequences from the C. morifolium genome. Using the luminescence as a visible marker, we demonstrated that inoculation of Agrobacterium harboring hyperactive piggyBac transposase removes inserted 2.6 kb DNA, which harbors piggyBac recognition sequences, from the modified Eluc sequence.
期刊介绍:
Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.