印度尼西亚日惹一个小型奶牛场在不同挤奶系统下获得的羊奶的微生物质量

Q2 Agricultural and Biological Sciences
Ismiarti ., Yustina Yuni Suranindya, Widodo .
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引用次数: 1

摘要

背景与目的:在印度尼西亚,小农奶牛场贡献了20.37%的国内市场。这些农场中有一小部分来自山羊奶牛场,这些农场传统上在挤奶时卫生条件差,储存管理不当。该系统会造成污染,影响消费者健康并造成经济损失。本研究旨在评价在印度尼西亚日惹的一个小农奶牛场在不同挤奶系统下获得的羊奶的微生物质量。材料与方法:选取杂交奶山羊20只,分为A组和b组。A组采用人工挤奶。B组采用斗式挤奶机挤奶。评估总平板计数(TPC)和肠杆菌科(EB)、产志贺毒素的大肠杆菌(STEC)和沙门氏菌的存在。所得数据采用t检验进行分析。结果:A组TPC和EB高于B组。A、B组产志贺毒素大肠杆菌占80%(8/10),沙门氏菌占30%(3/10)。系统发育分析表明,利用stx1引物扩增的基因组DNA部分测序结果与大肠杆菌O157:H7菌株志贺毒素亚基1A (stx1A)和志贺毒素亚基1B (stx1B)的多个序列具有90%以上的相似性。此外,使用16S rRNA引物对扩增的基因组DNA进行部分测序,与肠球菌的多个序列相似性超过90%。结论:小农奶山羊养殖场的卫生条件仍然较差,主要表现为病原菌的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiological Qualities of Goat Milk Obtained under Different Milking Systems at a Smallholder Dairy Farm in Yogyakarta, Indonesia
Background and Objective: In Indonesia, smallholder dairy farms contribute 20.37% of domestic market. A small portion of these farms is from goat dairy farms that are traditionally managed with poor sanitation during milking and improper storage management. This system causes contamination that can affect consumer health and cause financial loss. This study aimed to evaluate the microbiological quality of goat milk obtained under different milking systems at a smallholder dairy farm in Yogyakarta, Indonesia. Materials and Methods: Samples were collected from 20 crossbred dairy goats divided into two groups: Group A and B. In Group A, milking was conducted manually. In Group B, milking was conducted using a bucket milking machine. Total Plate Count (TPC) and the presence of Enterobacteriaceae (EB), Shiga toxin-producing Escherichia coli (STEC) and Salmonella were assessed. Data obtained were analyzed using a t-test. Results: The results showed that TPC and EB were higher in Group A than in Group B. In Groups A and B, 80% (8/10) of samples contained STEC and 30% (3/10) contained Salmonella. Phylogenetic analysis showed that partial sequencing of amplified genomic DNA using stx1 primers had more than 90% similarity with several sequences of Escherichia coli O157:H7 strain Shiga toxin subunit 1A (stx1A) and Shiga toxin subunit 1B (stx1B). Moreover, partial sequencing of amplified genomic DNA using 16S rRNA primers had more than 90% similarity with several sequences of S. enterica. Conclusion: The results conclude that hygienic and sanitary practices in smallholder dairy goat farming are still poor as shown by the presence of pathogenic bacteria.
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来源期刊
International Journal of Dairy Science
International Journal of Dairy Science Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
3.40
自引率
0.00%
发文量
7
期刊介绍: International Journal of Dairy Science is dedicated to disseminate the international original research on all aspect of dairy science. International Journal of Dairy Science publishes original scientific research on all aspects of dairy science including: animal husbandry, the physiology, biochemistry and endocrinology of lactation, milk production, composition, preservation, processing and separation, biotechnology and food science, properties of milk proteins and other components, dairy products such as cheese, fermented milks and spreads, relevant studies in bacteriology, enzymology and immunology, the use of milk products in other foods; and the development of methods relevant to these subjects.
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