内皮素受体拮抗剂对大鼠胃肠运动损伤的保护作用

Q3 Medicine
Hanna Ługowska-Umer, A. Umer, K. Kuziemski, Łukasz Sein-Anand, R. Korolkiewicz
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引用次数: 2

摘要

内皮素(ET)受体拮抗剂:BQ-123(ETA)、BQ-788(ETB)、替佐森坦(双重ET受体拮抗剂)可预防缺血再灌注(I/R)引起的术后肠梗阻(POI)的发展。目前的实验探讨了ET拮抗剂是否能预防外科肠道操作引起的POI的发生。通过测量乙醚麻醉大鼠在皮肤切开(SI)、剖腹手术(L)或剖腹手术和手术肠道处理(L+M)后的染料迁移率来评估肠道转运(E)。根据手术后恢复的时间,实验动物被随机分为两组:即2组或24组 h(早期或晚期POI)。E和SI不影响胃肠道(GI)转运。相反,与未治疗组(UN)相比,L和L+M显著降低了胃肠道运动。Tezosentan(10 mg/kg)、BQ-123和BQ-788(1 mg/kg)在晚期但不在早期POI过程中对L+M诱发的肠运动抑制的发展起保护作用。此外,替佐森坦在体外减轻了L+M诱导的纵向空肠平滑肌条对卡巴胆碱收缩反应的降低。与对照动物相比,术后早期或晚期POI组的血清ET(1-21)浓度均未增加。这项研究表明,手术诱导的POI后期肠道转运的延迟涉及ET依赖性机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The protective effect of endothelin receptor antagonists against surgically induced impairment of gastrointestinal motility in rats
Endothelin (ET) receptor antagonists: BQ-123 (ETA), BQ-788 (ETB), tezosentan (dual ET receptor antagonist) protect against the development of postoperative ileus (POI) evoked by ischemia-reperfusion (I/R). The current experiments explored whether ET antagonists prevent the occurrence of POI evoked by surgical gut manipulation. Intestinal transit was assessed by measuring the rate of dye migration subsequent to skin incision (SI), laparotomy (L), or laparotomy and surgical gut handling (L+M) in diethyl ether anaesthesized rats (E). Experimental animals were randomly sub-divided into two groups depending on the time of recovery following surgery: viz. either 2 or 24 h (early or late phase POI). E and SI did not affect the gastrointestinal (GI) transit. In contrast, L and L+M significantly reduced GI motility in comparison to untreated group (UN). Tezosentan (10 mg/kg), BQ-123 and BQ-788 (1 mg/kg) protected against development of L+M evoked inhibition of intestinal motility in the course of late phase, but not early phase POI. Furthermore, tezosentan alleviated the decrease in the contractile response of the longitudinal jejunal smooth muscle strips to carbachol in vitro induced by L+M. The serum ET(1–21) concentration was not increased in either the early or the late phase POI groups after surgery compared to control animals. This study indicates that delay in the intestinal transit in late phase of surgically induced POI involves an ET-dependent mechanism.
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来源期刊
Journal of Smooth Muscle Research
Journal of Smooth Muscle Research Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
2.30
自引率
0.00%
发文量
7
审稿时长
10 weeks
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