美洲棕榈叶提取物的抗菌、抗真菌和抗糖尿病作用。(樟科)

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Mercy Makopa, Benjamin Mangiza, Benjamin Banda, Winnie Mozirandi, Molly Mombeshora, S. Mukanganyama
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引用次数: 4

摘要

美洲波斯的果实和叶子被用于传统的医疗实践。本研究旨在研究美洲紫檀叶提取物的抗菌、抗真菌和抗糖尿病作用。测定了叶提取物对肺炎克雷伯菌和表皮葡萄球菌的抑菌活性,对白色念珠菌和热带念珠菌的抑菌活性。体外对α-葡萄糖苷酶进行抗糖尿病活性测定。采用微量肉汤稀释法考察微生物菌株对叶提取物的抑菌和抗真菌敏感性。表皮葡萄球菌是最敏感的微生物。丙酮提取物对表皮葡萄球菌的抑制作用最强,最低抑制浓度(MIC)为50 μg/mL。在100 μg/mL浓度下,18%的肺炎克雷伯菌细胞仍能存活。二氯甲烷(DCM)和甲醇提取物对白色念珠菌和热带念珠菌的活性分别为28%和8%。甲醇和丙酮提取物对表皮葡萄球菌的膜损伤表现为蛋白质渗漏。只有丙酮提取物对核酸泄漏有影响。对硝基苯酚-葡萄糖苷(底物)在405nm波长下生成对硝基酚,用分光光度法筛选提取物抑制α-葡萄糖苷酶活性的潜力。结果表明,甲醇提取物对α-葡萄糖苷酶的抑制作用最佳,且具有时间依赖性和剂量依赖性。孵育1小时后,kiandkinactvalue分别为1.4 mg/mL和2.4 U/min。结果表明,美洲蓟叶提取物含有抗菌、抗真菌和α-葡萄糖苷酶抑制作用的植物化学物质。需要进一步的研究来鉴定叶提取物中产生这些观察到的效果的活性化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae)
Fruits and leaves of Persia americana are used in traditional medical practices. This study was carried out to determine the antibacterial, antifungal, and antidiabetic effects of the leaf extracts from P. americana. The antibacterial activities of the leaf extracts were evaluated against Klebsiella pneumoniae and Staphylococcus epidermidis while antifungal activities were determined against Candida albicans and Candida tropicalis. The antidiabetic potential of the extracts was determined against mammalian α-glucosidase in vitro. The broth microdilution method was used to investigate the antibacterial and antifungal susceptibility of the microbial strains towards the leaf extracts. S. epidermidis was the most susceptible microbe out of the tested microorganisms. The acetone extract was the most potent extract against S. epidermidis with a minimum inhibitory concentration (MIC) of 50 μg/mL. At 100 μg/mL, the ethanol:water extract 18% of K. pneumoniae cells remained viable. Cell viability after exposure to the dichloromethane (DCM) and methanol extracts was 28% against C. albicans and 8% against C. tropicalis, respectively. The DCM:methanol and acetone extracts caused membrane damage in S. epidermidis exhibited by protein leakage. Only the acetone extract effected nucleic acid leakage. Screening of extracts’ potential to inhibit the activity of α-glucosidase was carried out spectrophotometrically following the production of p-nitrophenol from p-nitrophenol-glucopyranoside (substrate) at a wavelength of 405 nm. Out of all the tested extracts, the methanolic extract showed the best inhibitory activity on α-glucosidase enzyme in a time-dependent and dose-dependent manner.KiandKinactvalues were found to be 1.4 mg/mL and 2.4 U/min, respectively, after incubation for 1 hour. It was concluded that the leaf extracts of P. americana contain phytochemicals with antibacterial, antifungal, and α-glucosidase inhibitory effects. Further studies are required for the identification of the active compounds in the leaf extracts responsible for these observed effects.
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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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