Yuezhen Tian , Jianwen Du , Xuemei Yang , Weidan Zeng , Junmin He , Bingru Zhao , Xuefeng Fu , Xinming Xu , Weiwei Wu , Jiang Di , Xixia Huang , Kechuan Tian
{"title":"绵羊IGFBP2和IGFBP4启动子甲基化调节基因表达和毛囊发育","authors":"Yuezhen Tian , Jianwen Du , Xuemei Yang , Weidan Zeng , Junmin He , Bingru Zhao , Xuefeng Fu , Xinming Xu , Weiwei Wu , Jiang Di , Xixia Huang , Kechuan Tian","doi":"10.1016/j.ejbt.2022.07.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that <em>IGFBP2</em> and <em>IGFBP4</em> were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.</p></div><div><h3>Results</h3><p>The results showed that the expression levels of <em>IGFBP2</em> and <em>IGFBP4</em> in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for <em>IGFBP4</em> was significant. The total methylation rate of <em>IGFBP2</em> promoter was extremely low, and no significant difference was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of <em>IGFBP4</em> promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the <em>IGFBP4</em> were significantly negatively correlated with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of <em>IGFBP2</em> and <em>IGFBP4</em>, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.</p></div><div><h3>Conclusions</h3><p>The total methylation level of <em>IGFBP4</em> promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.</p><p><strong>How to cite:</strong> Tian Y, Du J, Yang X, et al. Sheep <em>IGFBP2</em> and <em>IGFBP4</em> promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.001.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 46-54"},"PeriodicalIF":2.3000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000276/pdfft?md5=73e08eec2be897991ff6de4818dffea1&pid=1-s2.0-S0717345822000276-main.pdf","citationCount":"3","resultStr":"{\"title\":\"Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development\",\"authors\":\"Yuezhen Tian , Jianwen Du , Xuemei Yang , Weidan Zeng , Junmin He , Bingru Zhao , Xuefeng Fu , Xinming Xu , Weiwei Wu , Jiang Di , Xixia Huang , Kechuan Tian\",\"doi\":\"10.1016/j.ejbt.2022.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that <em>IGFBP2</em> and <em>IGFBP4</em> were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.</p></div><div><h3>Results</h3><p>The results showed that the expression levels of <em>IGFBP2</em> and <em>IGFBP4</em> in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for <em>IGFBP4</em> was significant. The total methylation rate of <em>IGFBP2</em> promoter was extremely low, and no significant difference was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of <em>IGFBP4</em> promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the <em>IGFBP4</em> were significantly negatively correlated with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of <em>IGFBP2</em> and <em>IGFBP4</em>, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.</p></div><div><h3>Conclusions</h3><p>The total methylation level of <em>IGFBP4</em> promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.</p><p><strong>How to cite:</strong> Tian Y, Du J, Yang X, et al. Sheep <em>IGFBP2</em> and <em>IGFBP4</em> promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.001.</p></div>\",\"PeriodicalId\":11529,\"journal\":{\"name\":\"Electronic Journal of Biotechnology\",\"volume\":\"59 \",\"pages\":\"Pages 46-54\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0717345822000276/pdfft?md5=73e08eec2be897991ff6de4818dffea1&pid=1-s2.0-S0717345822000276-main.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electronic Journal of Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0717345822000276\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0717345822000276","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development
Background
Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that IGFBP2 and IGFBP4 were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.
Results
The results showed that the expression levels of IGFBP2 and IGFBP4 in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for IGFBP4 was significant. The total methylation rate of IGFBP2 promoter was extremely low, and no significant difference was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of IGFBP4 promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the IGFBP4 were significantly negatively correlated with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of IGFBP2 and IGFBP4, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.
Conclusions
The total methylation level of IGFBP4 promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.
How to cite: Tian Y, Du J, Yang X, et al. Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.001.
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
• Biofilms
• Bioinformatics
• Biomedicine
• Biopolicies of International Cooperation
• Biosafety
• Biotechnology Industry
• Biotechnology of Human Disorders
• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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