Seyed Morteza Robatjazi, Sanaz Mahboudi, M. Zeinoddini
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Production of Recombinant Denileukin Diftitox: Assessment in the Lab-scale Bioreactor
Materials and Methods: First, the effect of chemical composition in culture medium (the complex and defined) was investigated in DT389-IL2 expression in E.coli BL21 (DE3) containing pET-IDZ plasmid in shack flask and lab-bioreactor culture. To enrich the carbon source, we added glucose 6-8 g/L to the complex culture medium. The composition of the defined medium was enriched by adding amino acids (valine, 0.0502; phenylalanine, 0.0132; lysine, 0.0184; aspartic acid, 0.016; and serine, 0.0251 g/L). The growth cell was measured by the optical density method and the expression yield was investigated by SDS-PAGE.
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