{"title":"筛选到一个增强棉花耐盐性的黄酮醇合成酶(FLS)基因GhFLS1","authors":"Mingge Han, Ruifeng Cui, Yupeng Cui, Junjuan Wang, Shuai Wang, Tiantian Jiang, Hui Huang, Yuqian Lei, Xiaoyu Liu, Cun Rui, Yapeng Fan, Yuexin Zhang, Kesong Ni, Liangqing Sun, Xiugui Chen, Xuke Lu, Delong Wang, Zujun Yin, Chao Chen, Lixue Guo, Lanjie Zhao, Quanjia Chen, Wuwei Ye","doi":"10.1186/s12302-023-00743-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Flavonols play important roles in antioxidation and anticancer activities, longevity, and cardiovascular protection. <i>Flavonol synthase</i> (<i>FLS</i>) is a key enzyme for flavonol synthesis.</p><h3>Result</h3><p>Phenotypic, transcriptional and metabolic data were analyzed, which showed that there was a close relationship between salt stress and flavonoids, and flavonols were significantly upregulated under salt stress. Nine, seven, four, and four <i>FLS</i> genes were identified in <i>Gossypium hirsutum</i>, <i>Gossypium barbadense</i>, <i>Gossypium arboreum</i>, and <i>Gossypium raimondii,</i> respectively. The results of subcellular localization showed that FLS existed in the nucleus and cytoplasmic. Through phylogenetic analysis, 24 <i>FLS</i> genes were divided into three subfamilies. The results of the RNA sequencing showed that the expression of <i>GhFLS</i> genes was mainly induced by salt, drought, low temperature, and heat stress. <i>GhFLS</i> promoter mainly comprised plant hormone response elements and abiotic stress elements, indicating that the <i>GhFLS</i> gene may play a key role in abiotic stress response. The proline contents of pYL156:GhFLS1 was reduced significantly compared to pYL156 under salt stress, thereby reducing the resistance of cotton to salt stress.</p><h3>Conclusion</h3><p>This study will lay a foundation for further study on the antioxidant regulation mechanism of the <i>FLS</i> gene under abiotic stress.</p></div>","PeriodicalId":54293,"journal":{"name":"Environmental Sciences Europe","volume":"35 1","pages":""},"PeriodicalIF":5.9000,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enveurope.springeropen.com/counter/pdf/10.1186/s12302-023-00743-2","citationCount":"0","resultStr":"{\"title\":\"A flavonol synthase (FLS) gene, GhFLS1, was screened out increasing salt resistance in cotton\",\"authors\":\"Mingge Han, Ruifeng Cui, Yupeng Cui, Junjuan Wang, Shuai Wang, Tiantian Jiang, Hui Huang, Yuqian Lei, Xiaoyu Liu, Cun Rui, Yapeng Fan, Yuexin Zhang, Kesong Ni, Liangqing Sun, Xiugui Chen, Xuke Lu, Delong Wang, Zujun Yin, Chao Chen, Lixue Guo, Lanjie Zhao, Quanjia Chen, Wuwei Ye\",\"doi\":\"10.1186/s12302-023-00743-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Flavonols play important roles in antioxidation and anticancer activities, longevity, and cardiovascular protection. <i>Flavonol synthase</i> (<i>FLS</i>) is a key enzyme for flavonol synthesis.</p><h3>Result</h3><p>Phenotypic, transcriptional and metabolic data were analyzed, which showed that there was a close relationship between salt stress and flavonoids, and flavonols were significantly upregulated under salt stress. Nine, seven, four, and four <i>FLS</i> genes were identified in <i>Gossypium hirsutum</i>, <i>Gossypium barbadense</i>, <i>Gossypium arboreum</i>, and <i>Gossypium raimondii,</i> respectively. The results of subcellular localization showed that FLS existed in the nucleus and cytoplasmic. Through phylogenetic analysis, 24 <i>FLS</i> genes were divided into three subfamilies. The results of the RNA sequencing showed that the expression of <i>GhFLS</i> genes was mainly induced by salt, drought, low temperature, and heat stress. <i>GhFLS</i> promoter mainly comprised plant hormone response elements and abiotic stress elements, indicating that the <i>GhFLS</i> gene may play a key role in abiotic stress response. The proline contents of pYL156:GhFLS1 was reduced significantly compared to pYL156 under salt stress, thereby reducing the resistance of cotton to salt stress.</p><h3>Conclusion</h3><p>This study will lay a foundation for further study on the antioxidant regulation mechanism of the <i>FLS</i> gene under abiotic stress.</p></div>\",\"PeriodicalId\":54293,\"journal\":{\"name\":\"Environmental Sciences Europe\",\"volume\":\"35 1\",\"pages\":\"\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2023-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://enveurope.springeropen.com/counter/pdf/10.1186/s12302-023-00743-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Sciences Europe\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s12302-023-00743-2\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Sciences Europe","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1186/s12302-023-00743-2","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}
A flavonol synthase (FLS) gene, GhFLS1, was screened out increasing salt resistance in cotton
Background
Flavonols play important roles in antioxidation and anticancer activities, longevity, and cardiovascular protection. Flavonol synthase (FLS) is a key enzyme for flavonol synthesis.
Result
Phenotypic, transcriptional and metabolic data were analyzed, which showed that there was a close relationship between salt stress and flavonoids, and flavonols were significantly upregulated under salt stress. Nine, seven, four, and four FLS genes were identified in Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum, and Gossypium raimondii, respectively. The results of subcellular localization showed that FLS existed in the nucleus and cytoplasmic. Through phylogenetic analysis, 24 FLS genes were divided into three subfamilies. The results of the RNA sequencing showed that the expression of GhFLS genes was mainly induced by salt, drought, low temperature, and heat stress. GhFLS promoter mainly comprised plant hormone response elements and abiotic stress elements, indicating that the GhFLS gene may play a key role in abiotic stress response. The proline contents of pYL156:GhFLS1 was reduced significantly compared to pYL156 under salt stress, thereby reducing the resistance of cotton to salt stress.
Conclusion
This study will lay a foundation for further study on the antioxidant regulation mechanism of the FLS gene under abiotic stress.
期刊介绍：
ESEU is an international journal, focusing primarily on Europe, with a broad scope covering all aspects of environmental sciences, including the main topic regulation.
ESEU will discuss the entanglement between environmental sciences and regulation because, in recent years, there have been misunderstandings and even disagreement between stakeholders in these two areas. ESEU will help to improve the comprehension of issues between environmental sciences and regulation.
ESEU will be an outlet from the German-speaking (DACH) countries to Europe and an inlet from Europe to the DACH countries regarding environmental sciences and regulation.
Moreover, ESEU will facilitate the exchange of ideas and interaction between Europe and the DACH countries regarding environmental regulatory issues.
Although Europe is at the center of ESEU, the journal will not exclude the rest of the world, because regulatory issues pertaining to environmental sciences can be fully seen only from a global perspective.
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