R. Osei, Chengde Yang, Lingxiao Cui, Lijuan Wei, M. Jin, Solomon Boamah
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Co-culture of L. amnigena and SA significantly reduced the activity of pectinase, protease, pectin lyase and cellulase by an average of 33.8%, 43.4%, 67.7% and 46.9%, across the four concentrations (0.5 mM, 1.0 mM, 1.5 mM and 2.0 mM), respectively, compared to the control. The average disease index was reduced by 54.7% across the four SA concentrations. Treatment with SA induced transcriptional levels of the superoxide dismutase, peroxide, catalase and glutathione S-transferase across the four levels by an average of 3.87, 3.25, 3.97 and 3.94-fold, respectively, compared to control. 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The average disease index was reduced by 54.7% across the four SA concentrations. Treatment with SA induced transcriptional levels of the superoxide dismutase, peroxide, catalase and glutathione S-transferase across the four levels by an average of 3.87, 3.25, 3.97 and 3.94-fold, respectively, compared to control. 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引用次数: 4
摘要
摘要水杨酸(SA)在保护植物免受生物胁迫方面发挥着重要作用。遗忘型Lelliottia amnigena是一种新发现的马铃薯软腐病病原,目前尚未对其进行充分的研究。因此,本文重点研究SA对遗忘乳杆菌引起马铃薯软腐机理的影响。检测并检测了遗忘乳杆菌分泌果胶酶、蛋白酶、果胶裂解酶和纤维素酶,这是参与植物病害产生的最重要的致病酶。用0.2 mL amnigena乳杆菌悬浮液(3.69 CFU·mL−1×107 CFU·mL−1)接种灭菌的健康马铃薯块茎。24小时后,使用200μL的四种不同SA浓度(0.5 mM、1.0 mM、1.5 mM和2.0 mM)处理块茎。与对照相比,在四种浓度(0.5mM、1.0mM、1.5mM和2.0mM)下,遗忘乳杆菌和SA的共培养显著降低了果胶酶、蛋白酶、果胶裂解酶和纤维素酶的活性,平均分别降低了33.8%、43.4%、67.7%和46.9%。在四种SA浓度下,平均疾病指数降低了54.7%。与对照相比,SA处理诱导超氧化物歧化酶、过氧化物、过氧化氢酶和谷胱甘肽S-转移酶在四个水平上的转录水平分别平均提高3.87、3.25、3.97和3.94倍。根据我们的研究结果,我们可以说SA可以通过调节酶和非酶抗氧化活性以及诱导植物对细菌感染的自然抗性的基因表达来降低遗忘乳杆菌产生的这些胞外酶的活性。
Salicylic acid effect on the mechanism of Lelliottia amnigena causing potato soft rot
Abstract Salicylic acid (SA) plays an important role in protecting plants from biotic stresses. Lelliottia amnigena is a newly identified potato soft rot pathogen and there are no adequate studies on this soft rot pathogen. Therefore, this paper focussed on the effect of SA on the mechanism under which L. amnigena causes potato soft rot. L. amnigena was examined and detected to secrete pectinase, proteases, pectin lyase and cellulase, which are the most important pathogenic enzymes involved in the production of plant diseases. Sterilised healthy potato tubers were inoculated with 0.2 mL of L. amnigena suspension (3.69 CFU · mL−1 × 107 CFU · mL−1). After 24 h, 200 μL of four different SA concentrations (0.5 mM, 1.0 mM, 1.5 mM and 2.0 mM) were used to treat the tubers. Co-culture of L. amnigena and SA significantly reduced the activity of pectinase, protease, pectin lyase and cellulase by an average of 33.8%, 43.4%, 67.7% and 46.9%, across the four concentrations (0.5 mM, 1.0 mM, 1.5 mM and 2.0 mM), respectively, compared to the control. The average disease index was reduced by 54.7% across the four SA concentrations. Treatment with SA induced transcriptional levels of the superoxide dismutase, peroxide, catalase and glutathione S-transferase across the four levels by an average of 3.87, 3.25, 3.97 and 3.94-fold, respectively, compared to control. Based on our results, we could state that SA could reduce the activities of these extracellular enzymes produced by L. amnigena by modulating both enzymatic and non-enzymatic antioxidant activities and gene expression that induce natural resistance in plants against bacterial infections.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.