Homeobox D9通过负调控激肽释放酶相关肽酶9促进非小细胞肺癌的恶性进展

IF 2.9 4区 医学 Q1 Medicine
Juntao Hao, Zengqiang Shen, Junjun Ma
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引用次数: 0

摘要

我们研究了HOXD9在非小细胞肺癌(NSCLC)中的表达与预后的关系,并探讨了其在肿瘤进展中的潜在机制。采用qRT-PCR检测42对NSCLC组织样本中HOXD9的表达,并通过慢病毒载体转染评估其在NSCLC细胞系中的作用,采用CCK-8和Transwell检测评估细胞生长和迁移。生物信息学分析发现KLK9是HOXD9的靶基因,并在体内和体外研究了它们的相互作用。HOXD9 mRNA在NSCLC组织中的表达明显高于癌旁组织,与转移发生率和预后不良呈正相关。HOXD9敲低可降低NSCLC细胞的迁移和增殖,而过表达则相反。荧光素酶测定证实HOXD9与KLK9结合,导致KLK9表达负调控。KLK9的下调逆转了HOXD9对NSCLC细胞迁移和增殖的抑制作用,而KLK9和HOXD9的共过表达则起到相反的作用。我们的研究结果表明,HOXD9在NSCLC组织中的高表达与转移发生率和不良预后密切相关。HOXD9可能通过负调控KLK9在NSCLC中发挥其生物学功能。这些结果为推动NSCLC进展的分子机制提供了有价值的见解,突出了HOXD9作为潜在的预后生物标志物和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Homeobox D9 Promotes Malignant Progression of Non-Small Cell Lung Cancer Through Negatively Regulating Kallikrein Related Peptidase 9
We investigated the association between HOXD9 expression and prognosis in non-small cell lung cancer (NSCLC) and explored its underlying mechanisms in tumor progression. HOXD9 expression was examined in 42 pairs of NSCLC tissue samples using qRT-PCR, and its effects were evaluated in NSCLC cell lines through lentiviral vector transfection, assessing cell growth and migration using CCK-8 and Transwell assays. Bioinformatics analysis identified KLK9 as a target gene of HOXD9, and their interaction was investigated in vitro and in vivo. HOXD9 mRNA expression was significantly higher in NSCLC tissues compared to adjacent tissues, positively correlating with metastasis incidence and poor prognosis. HOXD9 knockdown reduced NSCLC cell migration and proliferation, while overexpression had the opposite effect. Luciferase assays confirmed HOXD9′s binding to KLK9, leading to negative regulation of KLK9 expression. Knockdown of KLK9 reversed the inhibitory effect of HOXD9 on NSCLC cell migration and proliferation, while co-overexpression of KLK9 and HOXD9 had the opposite effect. Our findings demonstrate the close association of high HOXD9 expression in NSCLC tissues with metastasis incidence and poor prognosis. HOXD9 likely exerts its biological function in NSCLC by negatively regulating KLK9. These results provide valuable insights into the molecular mechanisms driving NSCLC progression, highlighting HOXD9 as a potential prognostic biomarker and therapeutic target.
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来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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