墨西哥番茄变种MYB转录因子和靶基因的表达及其与酚类含量和抗氧化活性的关系

IF 2.1 4区农林科学

Cyta-Journal of Food Pub Date : 2022-11-16 DOI:10.1080/19476337.2022.2144951

Karen V. Pineda-Hidalgo, Brianda Flores-Leyva, Nancy Y. Salazar-Salas, Jeanett Chávez-Ontiveros, J. A. Garzón-Tiznado, Jorge Sánchez-López, F. Delgado-Vargas, J. López-Valenzuela

{"title":"墨西哥番茄变种MYB转录因子和靶基因的表达及其与酚类含量和抗氧化活性的关系","authors":"Karen V. Pineda-Hidalgo, Brianda Flores-Leyva, Nancy Y. Salazar-Salas, Jeanett Chávez-Ontiveros, J. A. Garzón-Tiznado, Jorge Sánchez-López, F. Delgado-Vargas, J. López-Valenzuela","doi":"10.1080/19476337.2022.2144951","DOIUrl":null,"url":null,"abstract":"ABSTRACT Eight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and CHI) by RT-qPCR. The AC (mmol TE/kg fw; TE, Trolox Equivalents) by ABTS (5.27–11.81), DPPH (1.43–3.67), and FRAP (4.41–9.22) correlated with TP (0.53–1.20 g GAE/kg fw; GAE, Gallic Acid Equivalents), TF (1.03–2.10 g QE/kg; QE, Quercetin Equivalents), and the levels of chlorogenic and dicaffeoylquinic acids and rutin. The expression of MYB12 and MYB14 correlated with that of PAL and CHS, while CHI was only associated with MYB12. The accessions Tumbisca and Kilim showed the highest gene expression, phenolics content, and AC, suggesting they can be used in breeding programs to produce tomatoes with better AC.","PeriodicalId":49084,"journal":{"name":"Cyta-Journal of Food","volume":"20 1","pages":"374 - 384"},"PeriodicalIF":2.1000,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico\",\"authors\":\"Karen V. Pineda-Hidalgo, Brianda Flores-Leyva, Nancy Y. Salazar-Salas, Jeanett Chávez-Ontiveros, J. A. Garzón-Tiznado, Jorge Sánchez-López, F. Delgado-Vargas, J. López-Valenzuela\",\"doi\":\"10.1080/19476337.2022.2144951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Eight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and CHI) by RT-qPCR. The AC (mmol TE/kg fw; TE, Trolox Equivalents) by ABTS (5.27–11.81), DPPH (1.43–3.67), and FRAP (4.41–9.22) correlated with TP (0.53–1.20 g GAE/kg fw; GAE, Gallic Acid Equivalents), TF (1.03–2.10 g QE/kg; QE, Quercetin Equivalents), and the levels of chlorogenic and dicaffeoylquinic acids and rutin. The expression of MYB12 and MYB14 correlated with that of PAL and CHS, while CHI was only associated with MYB12. The accessions Tumbisca and Kilim showed the highest gene expression, phenolics content, and AC, suggesting they can be used in breeding programs to produce tomatoes with better AC.\",\"PeriodicalId\":49084,\"journal\":{\"name\":\"Cyta-Journal of Food\",\"volume\":\"20 1\",\"pages\":\"374 - 384\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2022-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cyta-Journal of Food\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/19476337.2022.2144951\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cyta-Journal of Food","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/19476337.2022.2144951","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

摘要

摘要采用RT-qPCR技术对8份墨西哥番茄变种(Solanum lycopersicum var. cerasiforme)进行了总酚类物质(TP)和总黄酮(TF)、酚类物质谱(ulc - dad - ms)、抗氧化能力(AC) (ABTS、DPPH和FRAP)、转录因子(MYB12和MYB14)和靶基因(PAL、CHS和CHI)的表达进行了评价。AC (mmol TE/kg fw);ABTS(5.27 ~ 11.81)、DPPH(1.43 ~ 3.67)和FRAP(4.41 ~ 9.22)与TP (0.53 ~ 1.20 g GAE/kg fw)相关;GAE，没食子酸当量)，TF (1.03-2.10 g QE/kg;QE(槲皮素当量)，以及绿原酸、二咖啡酰奎宁酸和芦丁的含量。MYB12、MYB14的表达与PAL、CHS相关，CHI仅与MYB12相关。其中，Tumbisca和Kilim的基因表达量、酚类物质含量和AC含量最高，表明它们可以用于培育AC含量较高的番茄。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Expression of MYB transcription factors and target genes and its association with phenolic content and antioxidant activity of selected Solanum lycopersicum var. cerasiforme accessions from Mexico

ABSTRACT Eight Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) and flavonoids (TF), phenolic profiles (UPLC-DAD-MS), antioxidant capacity (AC) (ABTS, DPPH, and FRAP), and expression of transcription factors (MYB12 and MYB14) and target genes (PAL, CHS and CHI) by RT-qPCR. The AC (mmol TE/kg fw; TE, Trolox Equivalents) by ABTS (5.27–11.81), DPPH (1.43–3.67), and FRAP (4.41–9.22) correlated with TP (0.53–1.20 g GAE/kg fw; GAE, Gallic Acid Equivalents), TF (1.03–2.10 g QE/kg; QE, Quercetin Equivalents), and the levels of chlorogenic and dicaffeoylquinic acids and rutin. The expression of MYB12 and MYB14 correlated with that of PAL and CHS, while CHI was only associated with MYB12. The accessions Tumbisca and Kilim showed the highest gene expression, phenolics content, and AC, suggesting they can be used in breeding programs to produce tomatoes with better AC.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Cyta-Journal of Food FOOD SCIENCE & TECHNOLOGY-

CiteScore

4.40

自引率

0.00%

发文量

期刊介绍： CyTA – Journal of Food is an Open Access journal that publishes original peer-reviewed research papers dealing with a wide range of subjects which are essential to the food scientist and technologist. Topics include: chemical analysis of food; additives and toxins in food; sensory, nutritional and physiological aspects of food; food microbiology and biotechnology; changes during the processing and storage of foods; effect of the use of agrochemicals in foods; quality control in food; and food engineering and technology.