组蛋白甲基化酶EZH2和VEGF165在胸腺疾病中的作用

Zaiyu Guo, Heliang Zhang, Qian Chen, Yanwei Hou
{"title":"组蛋白甲基化酶EZH2和VEGF165在胸腺疾病中的作用","authors":"Zaiyu Guo, Heliang Zhang, Qian Chen, Yanwei Hou","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.005","DOIUrl":null,"url":null,"abstract":"Objective \nTo study on the role of histone methylation enzyme enhancer of zeste homolog 2 (EHZ2) and vascular endothelial growth factor 165 (VEGF165) in momymoya disease. \n \n \nMethods \nThe animal model of moyamoya disease was established by ear vein injection of horse serum in New Zealand rabbits. VEGF165 was over-expressed in situ by packaging lentivirus. Real-time quantitative PCR and Western Blot were used to detect the expression of VEGF165, EZH2 and H3K27me3 in the brain tissues of the animal models. \n \n \nResults \nCompared with the normal control group, the expression levels of mRNA and protein of EZH2 in the moyamoya disease model group were increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was increased. After overexpression of VEGF165 in the moyamoya disease model group, the expression levels of mRNA and protein of EZH2 was further increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was also increased. \n \n \nConclusions \nEZH2 plays a certain role in the pathogenesis of moyamoya disease, and the expression of EZH2 is regulated by VEGF 165, which provides a theoretical basis for the study of the pathogenesis of moyamoya disease. \n \n \nKey words: \nMoyamoya disease; Enhancer of zeste homolog 2; Vascular endothelial growth factor 165","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":"42 1","pages":"479-483"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Role of histone methylation enzyme EZH2 and VEGF165 in momymoya disease\",\"authors\":\"Zaiyu Guo, Heliang Zhang, Qian Chen, Yanwei Hou\",\"doi\":\"10.3760/CMA.J.ISSN.1673-4181.2019.06.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo study on the role of histone methylation enzyme enhancer of zeste homolog 2 (EHZ2) and vascular endothelial growth factor 165 (VEGF165) in momymoya disease. \\n \\n \\nMethods \\nThe animal model of moyamoya disease was established by ear vein injection of horse serum in New Zealand rabbits. VEGF165 was over-expressed in situ by packaging lentivirus. Real-time quantitative PCR and Western Blot were used to detect the expression of VEGF165, EZH2 and H3K27me3 in the brain tissues of the animal models. \\n \\n \\nResults \\nCompared with the normal control group, the expression levels of mRNA and protein of EZH2 in the moyamoya disease model group were increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was increased. After overexpression of VEGF165 in the moyamoya disease model group, the expression levels of mRNA and protein of EZH2 was further increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was also increased. \\n \\n \\nConclusions \\nEZH2 plays a certain role in the pathogenesis of moyamoya disease, and the expression of EZH2 is regulated by VEGF 165, which provides a theoretical basis for the study of the pathogenesis of moyamoya disease. \\n \\n \\nKey words: \\nMoyamoya disease; Enhancer of zeste homolog 2; Vascular endothelial growth factor 165\",\"PeriodicalId\":61751,\"journal\":{\"name\":\"国际生物医学工程杂志\",\"volume\":\"42 1\",\"pages\":\"479-483\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"国际生物医学工程杂志\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"国际生物医学工程杂志","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的探讨zeste同源物组蛋白甲基化酶增强子2 (EHZ2)和血管内皮生长因子165 (VEGF165)在肺癌中的作用。方法采用耳静脉注射马血清法建立新西兰兔烟雾病动物模型。通过包装慢病毒原位过表达VEGF165。采用实时定量PCR和Western Blot检测VEGF165、EZH2和H3K27me3在动物模型脑组织中的表达。结果与正常对照组比较,烟雾病模型组EZH2 mRNA及蛋白表达水平升高(P<0.01),组蛋白H3K27me3表达水平升高。过表达VEGF165后,烟雾病模型组EZH2 mRNA和蛋白表达水平进一步升高(EZH2 mRNA: P<0.01),组蛋白H3K27me3水平也升高。结论EZH2在烟雾病发病中发挥一定作用,EZH2的表达受VEGF 165的调控,为烟雾病发病机制的研究提供了理论依据。关键词:烟雾病;趣味同源物2的增强剂;血管内皮生长因子165
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of histone methylation enzyme EZH2 and VEGF165 in momymoya disease
Objective To study on the role of histone methylation enzyme enhancer of zeste homolog 2 (EHZ2) and vascular endothelial growth factor 165 (VEGF165) in momymoya disease. Methods The animal model of moyamoya disease was established by ear vein injection of horse serum in New Zealand rabbits. VEGF165 was over-expressed in situ by packaging lentivirus. Real-time quantitative PCR and Western Blot were used to detect the expression of VEGF165, EZH2 and H3K27me3 in the brain tissues of the animal models. Results Compared with the normal control group, the expression levels of mRNA and protein of EZH2 in the moyamoya disease model group were increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was increased. After overexpression of VEGF165 in the moyamoya disease model group, the expression levels of mRNA and protein of EZH2 was further increased (EZH2 mRNA: P<0.01), and the level of histone H3K27me3 was also increased. Conclusions EZH2 plays a certain role in the pathogenesis of moyamoya disease, and the expression of EZH2 is regulated by VEGF 165, which provides a theoretical basis for the study of the pathogenesis of moyamoya disease. Key words: Moyamoya disease; Enhancer of zeste homolog 2; Vascular endothelial growth factor 165
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
1974
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信