Y. E. Silina, E. V. Zolotukhina, M. Koch, C. Fink-Straube
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A tandem of GC-MS and electroanalysis for a rapid chemical profiling of bacterial extracellular matrix
Herein an assay toward a rapid and reliable profiling of extracellular matrix of Escherichia coli (E. coli) utilizing a tandem of GC-MS as a tool for definition of the exact chemical nature of low molecular weight compounds and cyclic voltammetry for their high throughput detection is presented.
Briefly, during a set of investigations the formation of glycerol in the extracellular matrix (ECM) of E. coli at physiological relevant conditions of cells was revealed. Based on the obtained knowledge, the electrochemical protocol allowing both qualitative and quantitative analyses of glycerol in E. coli ECMs at palladium ink-modified screen printed electrodes with precision values (RSD) <10 % and recovery rates ranged from 98 % to 102 % was proposed.
The provided protocol for a rapid electrochemical profiling of the bacterial ECMs can readily be used as a guideline for the controlled electroanalysis of target electroactive signaling analytes in complex biological samples.
期刊介绍：
Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications.
Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.
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