不同来源的胎儿胎盘组织冷冻提取物的组成和生物活性

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2023-03-30 DOI:10.15407/cryo33.01.003

M. Repin, Yu. A. Chizh, L. Marchenko, Tetyana Govorukha, Stanislav Narozhnyy

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引用次数: 0

摘要

采用凝胶渗透色谱法研究了大鼠胎儿组织（FTCE）和胎盘（PCE）冷冻提取物的蛋白质肽组成。使用冷冻-加温模式从组织匀浆中提取冷冻提取物：1-单一（-20°С）；2-双（-20；-196°С）和3-三（-20；–196；–196°С）。冷冻提取物的生物学活性通过完整大鼠在与金黄色葡萄球菌灭活培养物（2×109个细胞/ml）孵育45和120分钟后血液中中性粒细胞（NG）的吞噬活性进行体外评估，培养基中提取物的浓度为：1.6；3.1；以干物质计为6mg/ml。模式3的使用确保了冷冻提取物组合物中总蛋白质和蛋白质肽性质的低分子量化合物的更高产量。无论获得方式如何，FTCE中的低分子量组分含量都超过了PCE。NG与冷冻提取物的孵育并没有显著增加进入吞噬作用的中性粒细胞的数量。然而，与对照相比，PCE和FTCE的所有样品的特征是在45分钟孵育期间NG吸收活性的剂量依赖性增加。

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Composition and Biological Activity of Fetoplacental Tissues-Derived Cryoextracts Being Differently Obtained

The protein-peptide composition of fetal tissues (FTCEs) and placenta (PCE) cryoextracts of rats was investigated by gel permeation chromatography. Cryoextracts were derived from tissue homogenates using the freeze-warming modes: 1 – single (–20°С); 2 – double (–20; –196°С) and 3 – triple (–20; –196; –196°С) ones. The biological activity of cryoextracts was in vitro evaluated by the phagocytic activity of neutrophil granulocytes (NG) of the blood of intact rats after incubation with an inactivated culture of Staphylococcus aureus (2 × 109 cells/ml) for 45 and 120 min for the concentrations of extracts in the incubation medium: 1.6; 3.1; 6 mg/ml in terms of dry substance. The use of mode 3 ensured a greater yield of total protein and low molecular weight compounds of a protein-peptide nature in the composition of cryoextracts. The content of low-molecular-weight fractions in FTCE exceeded that in PCE, regardless of the obtaining mode. Incubation of NG with cryoextracts did not significantly increase the number of neutrophils which entered into phagocytosis. However, all the samples of PCE and FTCE were characterized by a dose-dependent rise in the NG absorption activity during the 45-minute incubation compared to the control.

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.