利用基因编码点击化学设计多功能蜘蛛丝

IF 18.5 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Bojing Jiang, Sin Yen Tan, Shiyu Fang, Xiaohan Feng, Byung Min Park, Hong Kiu Francis Fok, Zhongguang Yang, Ri Wang, Songzi Kou, Angela Ruohao Wu, Fei Sun
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引用次数: 1

摘要

蜘蛛丝以其卓越的机械性能和生物相容性而闻名,使其成为开发功能材料的多功能平台。在本研究中,使用基因编码点击化学的原型SpyTag/SpyCatcher化学,提出了重组蜘蛛丝的模块化功能化策略。该方法包括AlphaFold2辅助设计SpyTagged蜘蛛丝,结合细菌表达和仿生纺丝,使丝能够用各种SpyCatcher融合基序装饰,如荧光蛋白、酶和细胞结合配体。丝线可以使用silicatein(一种硅化酶)涂覆二氧化硅层,从而形成无机-有机混合1D材料。安装有RGD或层粘连蛋白细胞结合配体的线导致内皮细胞附着和增殖增强。这些发现展示了一种直接而强大的1D蛋白质材料方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry

Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry

Spider silk is recognized for its exceptional mechanical properties and biocompatibility, making it a versatile platform for developing functional materials. In this study, a modular functionalization strategy for recombinant spider silk is presented using SpyTag/SpyCatcher chemistry, a prototype of genetically encoded click chemistry. The approach involves AlphaFold2-aided design of SpyTagged spider silk coupled with bacterial expression and biomimetic spinning, enabling the decoration of silk with various SpyCatcher-fusion motifs, such as fluorescent proteins, enzymes, and cell-binding ligands. The silk threads can be coated with a silica layer using silicatein, an enzyme for silicification, resulting in a hybrid inorganic–organic 1D material. The threads installed with RGD or laminin cell-binding ligands lead to enhanced endothelial cell attachment and proliferation. These findings demonstrate a straightforward yet powerful approach to 1D protein materials.

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来源期刊
Advanced Functional Materials
Advanced Functional Materials 工程技术-材料科学:综合
CiteScore
29.50
自引率
4.20%
发文量
2086
审稿时长
2.1 months
期刊介绍: Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week. Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.
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