硫化氢介导的心肌缺血再灌注保护与线粒体保存的KATP通道有关，但与其对纤维间线粒体的明显偏好无关

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2019-06-09 DOI:10.3329/BJP.V14I2.39890

P. Chandrasekaran, S. Ravindran, S. Boovarahan, G. Kurian

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引用次数: 2

摘要

研究表明，硫化氢对心肌缺血再灌注损伤的保护作用是通过维持纤维间线粒体的功能活性而不是肌层下线粒体的功能活性。在这项研究中，研究了在NaSH (H2S供体)介导的心脏保护过程中，KATP通道在调节线粒体亚群中的作用。缺血-再灌注前，通过Langendorff灌注仪对离体大鼠心脏进行线粒体KATP通道封闭剂格列本脲(10 μM)/开启剂二氮氧化物(0.8 mM)处理。结果显示，存在格列本脲的NaSH预处理可显著改善心脏恢复，纤维间线粒体和肌层下线粒体之间无显著差异。综上所述，靶向KATP通道可能不是靶向纤维间线粒体/肌下线粒体抗缺血再灌注损伤的良好选择。

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Hydrogen sulfide-mediated cardioprotection against ischemia reperfusion is linked to KATP channel for mitochondrial preservation but not for its distinct preference on interfibrillar mitochondria

Hydrogen sulfide has been shown to protect myocardium against ischemia-reperfusion injury by preserving interfibrillar mitochondria functional activi-ties than subsarcolemmal mitochondria. In this study, the role of the KATP channel in modulating the mitochondrial subpopulations during the cardioprotection mediated by NaSH (H2S donor) was investigated. Isolated rat hearts were treated with mitochondrial KATP channel closer glibenclamide (10 μM)/opener diazoxide (0.8 mM) via Langendorff perfusion apparatus before ischemia-reperfusion. The results showed that NaSH pre-conditioning in presence of glibenclamide significantly improved cardiac recovery without any significant difference between interfibrillar mitochondria and subsarcolemmal mitochondria. In conclusion, targeting KATP channel may not be good option to target interfibrillar mitochondria/subsarcolemmal mitochondria against ischemia-reperfusion injury.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.