与植物激素信号转导及拉比蓝莓花粉自交不亲和性相关的微小RNA的鉴定

IF 1.2 4区农林科学 Q3 HORTICULTURE

Journal of the American Society for Horticultural Science Pub Date : 2022-11-01 DOI:10.21273/jashs05143-21

Qingrou Yang, Yan Fu, Tingting Zhang, Shu Peng, Jie Deng

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引用次数: 0

摘要

与植物激素信号转导和自交不亲和性相关的微小RNA（miRNA）可能在xenia效应中发挥重要作用。然而，拉比蓝莓（Vaccinium ashei）在这一领域的相关研究仍然缺乏。在这项研究中，我们鉴定了miRNA，预测了它们的靶基因，对靶基因进行了功能富集分析，并筛选了与植物激素信号传导和自交不亲和相关的miRNA。共鉴定出491个miRNA，其中在拉比蓝莓品种Brightwell和Premier中分别发现了27个和67个已知miRNA以及274个和416个新miRNA。与“Premier”相比，“Brightwell”中有31个miRNA上调，62个miRNA下调。京都基因和基因组百科全书（KEGG）功能富集分析表明，预测的4985个靶基因参与氨基酸生物合成、植物-病原体相互作用和剪接体途径。在拉比叶蓝莓花粉中，共鉴定出10个、1个、1、5个、2个、5个和2个分别与生长素、细胞分裂素、赤霉素、脱落酸、乙烯、油菜素甾体和水杨酸信号传导相关的候选miRNA。进一步分析表明，novel_miR_49是与自交不亲和性相关的候选miRNA，其靶基因为maker-VaccDscaff21-snap-gene-21.37。此外，对novel_miR_49靶基因的KEGG富集分析表明，它们参与核糖体、氨酰基tRNA生物合成和糖基磷脂酰肌醇锚定生物合成途径。结果表明，拉比叶蓝莓花粉的微小RNA在与生长素、细胞分裂素、赤霉素、脱落酸、乙烯、油菜素甾体和水杨酸相关的信号传导基础上，调节植物激素信号传导和自交不亲和信号传导。结果表明，有必要进一步研究miRNA对激素信号转导调控和自交不亲和性的影响，以阐明xenia效应的分子机制。

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Identification of MicroRNAs Related to Phytohormone Signal Transduction and Self-incompatibility of Rabbiteye Blueberry Pollen

MicroRNAs (miRNAs) related to phytohormone signal transduction and self-incompatibility may play an important role in the xenia effect. However, associated research in this area is still lacking in rabbiteye blueberry (Vaccinium ashei). In this study, we identified miRNAs, predicted their target genes, performed functional enrichment analysis of the target genes, and screened for miRNAs related to phytohormone signaling and self-incompatibility. A total of 491 miRNAs were identified, of which 27 and 67 known miRNAs as well as 274 and 416 new miRNAs were found in the rabbiteye blueberry cultivars Brightwell and Premier, respectively. Compared with ‘Premier’, 31 miRNAs were upregulated and 62 miRNAs were downregulated in ‘Brightwell’. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis indicated that the 4985 target genes predicted were involved in biosynthesis of amino acids, plant–pathogen interaction, and spliceosome pathways. A total of 10, one, one, five, two, five, and two candidate miRNAs related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling, respectively, in rabbiteye blueberry pollen were identified. Further analysis indicated that novel_miR_49 was a candidate miRNA related to self-incompatibility, and their target gene was maker-VaccDscaff21-snap-gene-21.37. In addition, the KEGG enrichment analysis of the target genes of novel_miR_49 showed that they were involved in the ribosome, aminoacyl-tRNA biosynthesis, and glycosylphosphatidylinositol-anchor biosynthesis pathways. The results revealed that the microRNAs of rabbiteye blueberry pollen regulated to phytohormone signal transduction and self-incompatibility signal transduction based on related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling. Results suggest that more research of the effects of miRNAs on regulation of hormone signal transduction and self-incompatibility is necessary for elucidating the molecular mechanism of the xenia effect.

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来源期刊

Journal of the American Society for Horticultural Science 农林科学-园艺

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The Journal of the American Society for Horticultural Science publishes papers on the results of original research on horticultural plants and their products or directly related research areas. Its prime function is to communicate mission-oriented, fundamental research to other researchers. The journal includes detailed reports of original research results on various aspects of horticultural science and directly related subjects such as: - Biotechnology - Developmental Physiology - Environmental Stress Physiology - Genetics and Breeding - Photosynthesis, Sources-Sink Physiology - Postharvest Biology - Seed Physiology - Postharvest Biology - Seed Physiology - Soil-Plant-Water Relationships - Statistics