利用高静水压力作为非生物激发剂提高辣椒自由和固定化细胞悬浮培养辣椒素的产量。

IF 1.2 4区物理与天体物理 Q3 PHYSICS, MULTIDISCIPLINARY

High Pressure Research Pub Date : 2021-03-24 DOI:10.1080/08957959.2021.1903458

C. İşlek, E. Koç, Ergin Murat Altuner, H. Alpas

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引用次数: 0

摘要

摘要本研究研究了不同施用时间固定化和高静水压力(HHP)对辣椒种子悬浮培养中辣椒素产量的影响。用离体萌发的辣椒幼苗下胚轴外植体进行愈伤组织培养，制备细胞悬浮液。利用这些细胞悬浮液可获得海藻酸钙固定化细胞悬浮培养物和游离细胞悬浮培养物。50、100、200、300和400 MPa分别作用于游离和固定细胞悬液作为激发剂。结果表明，在400 MPa压力下，第10天固定化细胞悬液中辣椒素含量最高，为293.187µg/g f.w。综上所述，固定化与激发剂联合施用可显著提高辣椒素的含量(p < 0.05)。

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Using high hydrostatic pressure as an abiotic elicitor strategy for improving capsaicin production in free and immobilized cell suspension cultures of Capsicum annuum L.

ABSTRACT In this study, the effects of immobilization and high hydrostatic pressure (HHP) on capsaicin production in cell suspension culture of pepper seeds at different application times were investigated. Callus cultures were obtained from in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from calluses. Immobilized cell suspension cultures with calcium alginate and free cell suspension cultures were obtained by using these cell suspensions. 50, 100, 200, 300 and 400 MPa were applied to both free and immobilized cell suspensions as an elicitor. When all the results were compared, the highest amount of capsaicin was achieved in immobilized cell suspensions on the tenth day as 293.187 µg/g f.w at 400 MPa pressure. As a result, it can be concluded that the combined application of immobilization and elicitor (HHP) caused significant increases (p < .05) in the amount of capsaicin.

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来源期刊

High Pressure Research 物理-物理：综合

CiteScore

3.80

自引率

5.00%

发文量

审稿时长

2 months

期刊介绍： High Pressure Research is the leading journal for research in high pressure science and technology. The journal publishes original full-length papers and short research reports of new developments, as well as timely review articles. It provides an important forum for the presentation of experimental and theoretical advances in high pressure science in subjects such as: condensed matter physics and chemistry geophysics and planetary physics synthesis of new materials chemical kinetics under high pressure industrial applications shockwaves in condensed matter instrumentation and techniques the application of pressure to food / biomaterials Theoretical papers of exceptionally high quality are also accepted.