嗜食性弧菌JSS中的核黄素转运体

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology
I. Rodionova, Fereshteh Heidari Tajabadi, Zhongge Zhang, D. Rodionov, M. Saier Jr.
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引用次数: 3

摘要

药物/代谢产物转运蛋白超家族的ImpX转运蛋白首次被提出用于转运核黄素(RF;维生素B2),这是基于对黄素单核苷酸(FMN核糖开关)有反应的顺式调节RNA元件的发现。外食Bdellovibrio JSS有一个同源物属于这个超科。它具有10个TMS,并且显示出与先前表征的来自有核梭杆菌的ImpX转运蛋白30%的同一性。然而,ImpX同源物不受FMN核糖开关的调节。为了测试来自外食B.ImpX同源物(BexImpX)的假定功能,我们克隆并异源表达了其基因。我们使用了功能互补、生长抑制实验、直接摄取实验和抑制研究,表明RF摄取具有高度特异性。RF生长的EC50估计在0.5–1µM范围内,根据支持RF营养缺陷型大肠杆菌菌株生长的最大RF浓度的一半估计,但RF吸收的狭河道为20µM。输运实验表明,能量来源是质子动力,但NaCl刺激吸收。因此,ImpX家族成员能够吸收RF,不仅在RF原营养物种(如有核F.nucleum)中,而且在B2营养缺陷型物种(B.exoovorous)中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Riboflavin Transporter in Bdellovibrio exovorous JSS
The ImpX transporters of the drug/metabolite transporter superfamily were first proposed to transport riboflavin (RF; vitamin B2) based on findings of a cis-regulatory RNA element responding to flavin mononucleotide (an FMN riboswitch). Bdellovibrio exovorous JSS has a homolog belonging to this superfamily. It has 10 TMSs and shows 30% identity to the previously characterized ImpX transporter from Fusobacterium nucleatum. However, the ImpX homolog is not regulated by an FMN-riboswitch. In order to test the putative function of the ImpX homolog from B. exovorous (BexImpX), we cloned and heterologously expressed its gene. We used functional complementation, growth inhibition experiments, direct uptake experiments and inhibition studies, suggesting a high degree of specificity for RF uptake. The EC50 for growth with RF was estimated to be in the range 0.5–1 µM, estimated from the half-maximal RF concentration supporting the growth of a RF auxotrophic Escherichia coli strain, but the Khalf for RF uptake was 20 µM. Transport experiments suggested that the energy source is the proton motive force but that NaCl stimulates uptake. Thus, members of the ImpX family members are capable of RF uptake, not only in RF prototrophic species such as F. nucleatum, but also in the B2 auxotrophic species, B. exovorous.
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来源期刊
Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物
CiteScore
3.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.
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