磁共振快速信号增强代谢物在活细胞原子尺度监测中的应用

IF 6.1 Q1 CHEMISTRY, MULTIDISCIPLINARY
Dr. Yonghong Ding, Dr. Sergey Korchak, Dr. Salvatore Mamone, Dr. Anil P. Jagtap, Dr. Gabriele Stevanato, Sonja Sternkopf, Denis Moll, Henning Schroeder, Dr. Stefan Becker, Prof. Dr. André Fischer, Dr. Ellen Gerhardt, Prof. Dr. Tiago F. Outeiro, Dr. Felipe Opazo, Prof. Dr. Christian Griesinger, Dr. Stefan Glöggler
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引用次数: 12

摘要

尽管核磁共振本身是一种不敏感的现象,但它在分析学和生物医学等领域有着广泛的应用。克服敏感性挑战是进一步扩大核磁共振适用性的关键,例如,改善医学诊断。在这里,我们提出了一种快速的策略,用对氢增强13c标记的代谢物的信号,特别是13c -丙酮酸,一个重要的能量代谢分子。我们成功地获得了1-13C-丙酮酸在水中平均27%的13C极化,这使我们能够引入研究细胞代谢的两个应用。首先,我们证明了1- 13c -丙酮酸的代谢可以作为帕金森病细胞模型的生物标志物,其次,我们引入了将同一细胞中的实时代谢分析与蛋白质结构测定相结合的机会。基于这里提出的结果,我们设想在未来的生物医学研究中使用我们的方法来检测疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Rapidly Signal-enhanced Metabolites for Atomic Scale Monitoring of Living Cells with Magnetic Resonance

Rapidly Signal-enhanced Metabolites for Atomic Scale Monitoring of Living Cells with Magnetic Resonance

Nuclear magnetic resonance (NMR) is widely applied from analytics to biomedicine although it is an inherently insensitive phenomenon. Overcoming sensitivity challenges is key to further broaden the applicability of NMR and, for example, improve medical diagnostics. Here, we present a rapid strategy to enhance the signals of 13C-labelled metabolites with para-hydrogen and, in particular, 13C-pyruvate, an important molecule for the energy metabolism. We succeeded to obtain an average of 27 % 13C polarization of 1-13C-pyruvate in water which allowed us to introduce two applications for studying cellular metabolism. Firstly, we demonstrate that the metabolism of 1-13C-pyruvate can serve as a biomarker in cellular models of Parkinson's disease and, secondly, we introduce the opportunity to combine real-time metabolic analysis with protein structure determination in the same cells. Based on the here presented results, we envision the use of our approach for future biomedical studies to detect diseases.

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CiteScore
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