5-氨基乙酰丙酸与微生物合成金纳米粒子的结合研究,评价其光动力治疗皮肤黑色素瘤和表皮样癌细胞系的作用

IF 2.2 4区 工程技术 Q2 Chemistry
S. Aishwarya, K. R. Sanjay
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引用次数: 11

摘要

几十年来,使用微创技术进行癌症治疗一直是一项挑战。在本研究中,光动力癌症治疗(PDT)的概念已被应用于生物合成纳米颗粒治疗皮肤黑色素瘤(B16F10)和表皮样癌(A431)细胞。生物合成的纳米颗粒与光敏剂药物5-氨基乙酰丙酸(5-ALA)结合,通过照射激活原卟啉IX (PpIX)的形成来治疗癌细胞。利用大肠杆菌菌株合成金纳米颗粒。采用扫描电镜、动态光散射和zeta电位分析研究了纳米颗粒的尺寸和特性。金纳米颗粒被聚乙二醇(PEG)包裹,并用光敏剂药物5-氨基乙酰丙酸标记。这种药物是用卤素灯激活的,以增强活性氧(ROS)分子的产生。研究人员评估了纯纳米颗粒和共轭纳米颗粒对皮肤黑色素瘤和表皮样癌细胞系的细胞毒性,并与标准药物5-ALA进行了比较,并使用2,7二氯荧光素-二乙酸酯(DCFH-DA)探针测量了ROS分子的产生。结果表明,与5-ALA和金-5-ALA缀合物相比,纯金纳米颗粒对细胞具有更大的细胞毒性。但当细胞受到辐照时,金-5-ALA缀合物表现出比5-ALA和纯纳米颗粒更高的细胞毒性。金-5- ala偶联物的细胞毒性水平增加了一倍,这表明与其他样品相比,产生了更多的活性氧分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Conjugation study of 5-aminolevulinic acid with microbial synthesized gold nanoparticles to evaluate its effect on skin melanoma and epidermoid carcinoma cell lines using photodynamic cancer therapy

Conjugation study of 5-aminolevulinic acid with microbial synthesized gold nanoparticles to evaluate its effect on skin melanoma and epidermoid carcinoma cell lines using photodynamic cancer therapy

Cancer therapy using minimal invasive technique has been a challenge since decades. In the present research, the concept of photodynamic cancer therapy (PDT) has been applied on biologically synthesized nanoparticles for the treatment of skin melanoma (B16F10) and epidermoid carcinoma (A431) cells. The biologically synthesized nanoparticles have been conjugated with a photosensitizer drug 5-aminolevulinic (5-ALA) acid to treat cancer cells by activating the protoporphyrin IX (PpIX) formation through irradiation. Bacterial strain Escherichia coli was used for the synthesis of gold nanoparticles. The size and characteristics of nanoparticles were studied with scanning electron microscope, dynamic light scattering and zeta potential analysis. The gold nanoparticles were encapsulated with polyethylene glycol (PEG) and tagged with 5-aminolevulinic acid, a photosensitizer drug. The drug was activated using a halogen lamp to enhance the production of reactive oxygen species (ROS) molecules. The cytotoxicity of pure nanoparticles and conjugated nanoparticles were assessed on skin melanoma and epidermoid carcinoma cell lines and compared against standard drug 5-ALA, and the production of ROS molecules was measured using a 2,7 dichlorofluorescein-diacetate (DCFH-DA) probe. The results indicated that pure gold nanoparticles had greater cytotoxicity on cells compared to 5-ALA and gold-5-ALA conjugate. But when the cells were subjected to irradiation, the gold-5-ALA conjugate showed higher cytotoxicity than 5-ALA and pure nanoparticles. The cytotoxic levels of gold-5-ALA conjugates were doubled which indicated greater reactive oxygen molecule production compared to other samples.

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来源期刊
Gold Bulletin
Gold Bulletin 工程技术-材料科学:综合
CiteScore
3.30
自引率
4.50%
发文量
0
审稿时长
3 months
期刊介绍: Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.
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