A. K. Yaqubi, S. D. Astuti, P. Permatasari, N. Komariyah, E. Endarko, A. Zaidan
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Statistical tests were utilized in data analysis, namely the One Way Anova test (analysis of variance). The results of this study indicated that 395 nm purple LED irradiation caused a decrease in Log CFU/mL of Bacillus subtilis and Escherichia coli bacteria. Inactivation of Bacillus subtilis bacteria showed a higher mortality percentage than Escherichia coli bacteria. Changes in other irradiation distances also showed a higher percentage of death for Bacillus subtilis bacteria than Escherichia coli bacteria. The highest percentage of death was 98.5% for Bacillus subtilis bacteria and 94.3% for Escherichia coli bacteria at position C with an irradiation distance of 3 cm and an energy density of 524 J/cm2 with an LED exposure time of 120 minutes. 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引用次数: 1
摘要
细菌的灭活使用一种称为光动力灭活的技术,该技术将光与具有正确光谱的光敏剂结合在一起。本研究的目的是确定紫色LED光灭枯草芽孢杆菌和大肠杆菌的效率以及理想的紫色LED照射能量密度。这项研究技术包括将细菌暴露在紫色LED辐射下。辐照过程中使用了两个变量。第一个变化是距离为3厘米、6厘米、9厘米和12厘米处的光照变化。第二种变化包括在30、60、90和120分钟内改变辐射量。采用总平板计数法(Total Plate Count, TPC)对菌落进行计数。数据分析采用统计检验,即One Way Anova检验(方差分析)。本研究结果表明,395 nm紫色LED照射可使枯草芽孢杆菌和大肠杆菌的Log CFU/mL减少。枯草芽孢杆菌的失活死亡率高于大肠杆菌。其他照射距离的变化也表明枯草芽孢杆菌的死亡率高于大肠杆菌。当LED照射时间为120分钟,照射距离为3 cm,能量密度为524 J/cm2时,C位置枯草芽孢杆菌和大肠杆菌的死亡率最高,分别为98.5%和94.3%。这表明枯草芽孢杆菌和大肠杆菌的死亡率随着LED能量剂量的增加而增加,其中革兰氏阳性细菌枯草芽孢杆菌的死亡率最高。
Effectiveness of purple led for inactivation of Bacillus subtilis and Escherichia coli bacteria in in vitro sterilizers
Bacteria are inactivated using a technique called photodynamic inactivation, which combines light with a photosensitizer with the right spectrum. The objective of this study is to ascertain the eciency of purple LEDs for photoinactivating Bacillus subtilis and Escherichia coli bacteria as well as the ideal purple LED exposure energy density. This study technique involves exposing bacteria to purple LED radiation. Two elements of variation are used during irradiation. The first variation is the illumination variation at distances of 3 cm, 6 cm, 9 cm, and 12 cm. The second variation involves changing the amount of radiation for 30, 60, 90, and 120 minutes. The Total Plate Count (TPC) method was used to count the number of colonies. Statistical tests were utilized in data analysis, namely the One Way Anova test (analysis of variance). The results of this study indicated that 395 nm purple LED irradiation caused a decrease in Log CFU/mL of Bacillus subtilis and Escherichia coli bacteria. Inactivation of Bacillus subtilis bacteria showed a higher mortality percentage than Escherichia coli bacteria. Changes in other irradiation distances also showed a higher percentage of death for Bacillus subtilis bacteria than Escherichia coli bacteria. The highest percentage of death was 98.5% for Bacillus subtilis bacteria and 94.3% for Escherichia coli bacteria at position C with an irradiation distance of 3 cm and an energy density of 524 J/cm2 with an LED exposure time of 120 minutes. This shows that the percentage of death of bacteria Bacillus subtilis and Escherichia coli increased with increasing doses of LED energy with the greatest percentage of death in Gram-positive bacteria Bacillus subtilis.
期刊介绍:
The main goal of the journal – to promote the development of Russian biomedical photonics and implementation of its advances into medical practice. The primary objectives: - Presentation of up-to-date results of scientific and in research and scientific and practical (clinical and experimental) activity in the field of biomedical photonics. - Development of united Russian media for integration of knowledge and experience of scientists and practitioners in this field. - Distribution of best practices in laser medicine to regions. - Keeping the clinicians informed about new methods and devices for laser medicine - Approval of investigations of Ph.D candidates and applicants.