中耳炎伴和不伴中耳炎伴积液的中耳腔菌群

IF 0.7 Q4 OTORHINOLARYNGOLOGY
R. Durmaz, B. Durmaz, O. Arı, Olkar Abdulmajed, Serdal Çelik, M. Kalcıoğlu
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引用次数: 1

摘要

目的:目前尚无资料揭示人类中耳腔真菌群落(mycobiome)的组成和多样性。本研究调查了健康中耳个体和中耳炎伴积液患者中耳腔中的真菌群。方法:1组47例(儿童35例,成人12例),2组20例(儿童20例),共采集77例中耳和4例腺样体标本。使用Illumina MiSeq宏基因组学试剂盒,利用基于核糖体内部转录间隔2 (ITS2)的元条形码分析真菌组谱。结果:基于its2的元条形码检测到14个属和17个种,平均相对丰度≥1%。腺样体组织和中耳腔、1组和2组、儿童和成人之间的真菌组谱相似。镰刀菌属、茎孢菌属、念珠菌属和枝孢菌属是所有样品中检出最多的属。2组假丝酵母菌属和镰刀菌属的平均相对丰度显著高于1组。结论:青绿假丝酵母、cretensis假丝酵母、橡胶曲霉、desertorpenicillium、arrhizopus在渗出性中耳炎(OME)患者中数量显著增加,可能影响OME的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mycobiome in the Middle Ear Cavity with and Without Otitis Media with Effusion
Objective: No data have yet been published revealing the composition and the diversity of fungal communities (mycobiome) in the human middle ear cavity. The presented study investigated the mycobiome in the middle ear cavities of individuals with healthy middle ears and patients with otitis media with effusion. Methods: A total of 77 middle ear and four adenoid samples were collected from 47 individuals (35 children and 12 adults) in Group 1 and from 20 children in Group 2. The mycobiome profile was analyzed with nuclear ribosomal internal transcribed spacer 2 (ITS2) based metabarcoding using an Illumina MiSeq metagenomics kit. Results: ITS2-based metabarcoding detected 14 different genera and 17 different species with a mean relative abundance of ≥1% in the samples analyzed. Mycobiome profile was similar between the adenoid tissue and the middle ear cavity, between Groups 1 and Group 2, and between children and adults. Fusarium, Stemphylium, Candida, and Cladosporium were the most abundant genera detected in all samples. The mean relative abundances of the genera Candida and Fusarium were remarkably higher in Group 2 compared to Group 1. Conclusion: The species Candida glaebosa, Candida cretensis, Aspergillus ruber, Penicillium desertorum, and Rhizopus arrhizus were significantly more abundant in patients with otitis media with effusion (OME), raising the possibility that they affect the pathogenesis of OME.
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