蛋白质翻译扩散作为一种检测分子间相互作用的方法

IF 4.9 Q1 BIOPHYSICS
Biophysical reviews Pub Date : 2023-08-16 eCollection Date: 2023-10-01 DOI:10.1007/s12551-023-01108-y
Yuriy F Zuev, Aleksandra M Kusova, Aleksandr E Sitnitsky
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引用次数: 0

摘要

在这项工作中,我们分析了从大分子扩散中获得的蛋白质分子间相互作用的信息。通过动态光散射和脉冲场梯度核磁共振(PFG NMR)光谱分析蛋白质的平移自扩散和集体扩散,利用Vink的非平衡热力学摩擦形式分析粒子的扩散运动,并利用Derjaguin-Landau-Verwey-Overbeek (DLVO)理论分析电解质中胶体粒子的相互作用,我们的方法给出了最有希望的结果解决方案。早期我们已经证明Vink理论与DLVO的结合为PFG NMR和DLS实验对扩散系数浓度依赖性的统一解释提供了可靠的基础。理论和数学程序的基本细节,并提出了各种结构形式,大小和形状的蛋白质提出的概念的实验证明的广泛分析。在本综述中,我们的方法的主要功能是获得不同形状、内部结构和质量的蛋白质分子间相互作用的细节。实验证明了Vink方法的通用性,对结构和形状复杂的蛋白质的实验结果给出了恰当的描述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein translational diffusion as a way to detect intermolecular interactions.

In this work, we analyze the information on the protein intermolecular interactions obtained from macromolecular diffusion. We have shown that the most hopeful results are given by our approach based on analysis of protein translational self-diffusion and collective diffusion obtained by dynamic light scattering and pulsed-field gradient NMR (PFG NMR) spectroscopy with the help of Vink's approach to analyze diffusion motion of particles by frictional formalism of non-equilibrium thermodynamics and the usage of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory of colloid particles interactions in electrolyte solutions. Early we have shown that integration of Vink's theory with DLVO provides a reliable basis for uniform interpreting of PFG NMR and DLS experiments on concentration dependence of diffusion coefficients. Basic details of theoretical and mathematical procedures and a broad analysis of experimental attestation of proposed conception on proteins of various structural form, size, and shape are presented. In the present review, the main capabilities of our approach obtain the details of intermolecular interactions of proteins with different shapes, internal structures, and mass. The universality of Vink's approach is experimentally shown, which gives the appropriate description of experimental results for proteins of complicated structure and shape.

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来源期刊
Biophysical reviews
Biophysical reviews Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
8.90
自引率
0.00%
发文量
93
期刊介绍: Biophysical Reviews aims to publish critical and timely reviews from key figures in the field of biophysics. The bulk of the reviews that are currently published are from invited authors, but the journal is also open for non-solicited reviews. Interested authors are encouraged to discuss the possibility of contributing a review with the Editor-in-Chief prior to submission. Through publishing reviews on biophysics, the editors of the journal hope to illustrate the great power and potential of physical techniques in the biological sciences, they aim to stimulate the discussion and promote further research and would like to educate and enthuse basic researcher scientists and students of biophysics. Biophysical Reviews covers the entire field of biophysics, generally defined as the science of describing and defining biological phenomenon using the concepts and the techniques of physics. This includes but is not limited by such areas as: - Bioinformatics - Biophysical methods and instrumentation - Medical biophysics - Biosystems - Cell biophysics and organization - Macromolecules: dynamics, structures and interactions - Single molecule biophysics - Membrane biophysics, channels and transportation
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