d -蒎醇对博莱霉素诱导大鼠肺纤维化的调节作用

IF 1.7 4区 医学 Q3 TROPICAL MEDICINE
Yuzhou Duan, Zhi-Hua Wang, Yanfei Huo, Yang Zhang, Xiao-Ran Wu, Cuike Gong, Lin-lin Bai
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引用次数: 0

摘要

目的:探讨d -匹尼醇对博来霉素所致肺纤维化的影响。方法:Sprague-Dawley大鼠气管内给予博来霉素(6 IU/kg)诱导肺纤维化,随后给予D-pinitol(5、10或20 mg/kg)或载药或甲基强的松龙(10 mg/kg) 28天。观察肺功能、生化指标、血清生化、mRNA表达及组织学特征。结果:10、20 mg/kg d -蒎醇能显著(P<0.05)减淡博莱霉素诱导的支气管肺泡灌洗液,降低髓过氧化物酶、一氧化氮、丙二醛水平,升高谷胱甘肽和超氧化物歧化酶水平。d -匹尼醇还能改善肺功能(增强暂停、呼吸频率、呼气量和潮气量)。D-pinitol显著上调Nrf2 (P<0.05),下调TGF-β、collagen-1、Smad-3 mRNA的表达。此外,D-pinitol组的炎症(支气管周围、血管周围和总炎症)、Ashcroft和间质纤维化评分明显降低。结论:d -蒎醇可通过抗氧化和抗纤维化途径抗博莱霉素所致肺纤维化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulatory effect of D-pinitol on bleomycin-induced pulmonary fibrosis in rats
Objective: To assess the effect of D-pinitol on pulmonary fibrosis induced by bleomycin. Methods: Sprague-Dawley rats received intratracheal bleomycin (6 IU/kg) to induce pulmonary fibrosis, followed by administration of either D-pinitol (5, 10, or 20 mg/kg) or vehicle or methylprednisolone (10 mg/kg) over 28 days after bleomycin administration. Lung function, biochemical parameters, serum biochemistry, mRNA expressions, and histological features were observed. Results: D-pinitol at 10 and 20 mg/kg significantly (P<0.05) attenuated bleomycin-induced bronchoalveolar lavage fluid, decreased myeloperoxidase, nitric oxide, malondialdehyde levels, and increased glutathione and superoxide dismutase level. D-pinitol also improved lung function (enhanced pause, frequency of breathing, expired volume, and tidal volume). Besides, D-pinitol significantly (P<0.05) upregulated Nrf2 and downregulated mRNA expressions of TGF-β, collagen-1, and Smad-3. Furthermore, considerably less inflammation (peribronchial, perivascular, and total), Ashcroft, and interstitial fibrosis scores were observed in the D-pinitol group. Conclusions: D-pinitol exerts its effect against bleomycin-induced pulmonary fibrosis via antioxidative and anti-fibrotic pathways.
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来源期刊
Asian Pacific journal of tropical biomedicine
Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.10
自引率
11.80%
发文量
2056
审稿时长
4 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.
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