Sun Zhanxue , Wang Jingjun , Kong Yuhong , Li Nan , Jiang Xiaoyuan , Cao Ting , Jia Yuanning , Zhang Yueyue , Zhang Yisheng , Cheng Jing
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Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction (RT-PCR) and immunoblotting, respectively.</p></div><div><h3>RESULTS</h3><p>Yupingfeng granule treatment resulted in significant acceleration of wound healing with 63.64% efficiency, which was significantly higher than that of placebo granule treatment (31.82%, <em>P</em> < 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ±31) <em>vs</em> (360 ±21) urn, <em>P</em> < 0.01], weight of the wounded skin [(42 ± 4) <em>vs</em> (24 ± 5) mg, <em>P</em> < 0.01], and TEWL [(30 ±4) <em>vs</em> (13 ± 4) g • h<sup>−1</sup> • m<sup>−2</sup>, <em>P</em> < 0.01]. Yupingfeng granules also significantly down-regulated mRNA and protein expression of AQP3 in the animal models.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggested that Yupingfeng granules could be used in AD treatment.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 872-878"},"PeriodicalIF":2.0000,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30986-5","citationCount":"5","resultStr":"{\"title\":\"Effect of Yupingfeng granules on the skin barrier in atopic dermatitis mice models\",\"authors\":\"Sun Zhanxue , Wang Jingjun , Kong Yuhong , Li Nan , Jiang Xiaoyuan , Cao Ting , Jia Yuanning , Zhang Yueyue , Zhang Yisheng , Cheng Jing\",\"doi\":\"10.1016/S0254-6272(18)30986-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate effect of Yupingfeng granules, prepared with Chinese Medicines, on the wound healing and on the expression of aquaporin 3 (AQP3) and the skin barrier in the animal models of atopic dermatitis (AD).</p></div><div><h3>METHODS</h3><p>Acute skin lesions of AD models were prepared using 2,4-dinitrochlorobenzo (DNCB) in mice and animals were treated with either Yupingfeng granules or placebo for two weeks. Skin wound healing outcome was assessed by measuring skin thickness, weight (quality) of the skin, and trans-epidermal water loss (TEWL). Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction (RT-PCR) and immunoblotting, respectively.</p></div><div><h3>RESULTS</h3><p>Yupingfeng granule treatment resulted in significant acceleration of wound healing with 63.64% efficiency, which was significantly higher than that of placebo granule treatment (31.82%, <em>P</em> < 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ±31) <em>vs</em> (360 ±21) urn, <em>P</em> < 0.01], weight of the wounded skin [(42 ± 4) <em>vs</em> (24 ± 5) mg, <em>P</em> < 0.01], and TEWL [(30 ±4) <em>vs</em> (13 ± 4) g • h<sup>−1</sup> • m<sup>−2</sup>, <em>P</em> < 0.01]. 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引用次数: 5
摘要
目的探讨中药复方玉屏风颗粒对特应性皮炎(AD)动物模型创面愈合、水通道蛋白3 (AQP3)表达及皮肤屏障的影响。方法采用2,4-二硝基氯苯(DNCB)制备AD小鼠急性皮损模型,动物分别给予玉坪风颗粒或安慰剂治疗2周。通过测量皮肤厚度、皮肤重量(质量)和经表皮水分损失(TEWL)来评估皮肤伤口愈合效果。采用逆转录聚合酶链反应(RT-PCR)和免疫印迹法分别检测AQP3 mRNA和蛋白的表达。结果九屏风颗粒治疗显著促进创面愈合,有效率为63.64%,显著高于安慰剂颗粒治疗的31.82%,P <Wilcoxon秩和检验0.01)。与正常动物相比,AD模型的皮肤厚度、损伤皮肤重量和TEWL均显著升高。玉屏风颗粒治疗后皮肤厚度显著降低[(937±31)vs(360±21);0.01],伤皮重量[(42±4)vs(24±5)mg, P <[0.01], TEWL[(30±4)vs(13±4)g•h−1•m−2,P <0.01]。玉屏风颗粒在动物模型中也能显著下调AQP3 mRNA和蛋白的表达。结论玉屏风颗粒可用于阿尔茨海默病的治疗。
Effect of Yupingfeng granules on the skin barrier in atopic dermatitis mice models
OBJECTIVE
To investigate effect of Yupingfeng granules, prepared with Chinese Medicines, on the wound healing and on the expression of aquaporin 3 (AQP3) and the skin barrier in the animal models of atopic dermatitis (AD).
METHODS
Acute skin lesions of AD models were prepared using 2,4-dinitrochlorobenzo (DNCB) in mice and animals were treated with either Yupingfeng granules or placebo for two weeks. Skin wound healing outcome was assessed by measuring skin thickness, weight (quality) of the skin, and trans-epidermal water loss (TEWL). Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction (RT-PCR) and immunoblotting, respectively.
RESULTS
Yupingfeng granule treatment resulted in significant acceleration of wound healing with 63.64% efficiency, which was significantly higher than that of placebo granule treatment (31.82%, P < 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ±31) vs (360 ±21) urn, P < 0.01], weight of the wounded skin [(42 ± 4) vs (24 ± 5) mg, P < 0.01], and TEWL [(30 ±4) vs (13 ± 4) g • h−1 • m−2, P < 0.01]. Yupingfeng granules also significantly down-regulated mRNA and protein expression of AQP3 in the animal models.
CONCLUSION
Our findings suggested that Yupingfeng granules could be used in AD treatment.
期刊介绍:
Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.