{"title":"杉木心材提取物在非织造布上的抗菌活性、稳定性和溶血活性","authors":"Tharathip Hemthanon, Pakpimol Ungcharoenwiwat","doi":"10.1016/j.ejbt.2021.10.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Antimicrobial substances from medicinal plants have been widely applied in many industries, without the concern of side effects. Nonwoven fabric products such as face masks, paper wipes, and wound dressings contain antibacterial substances for reduction of bacterial accumulation. Therefore, the use of bioactive compounds from medicinal plants improves the properties of fabrics. This study used a crude extract from <em>Caesalpinia sappan</em> to perform phytochemical screening and investigate its antibacterial activity, stability, hemolytic activity, and preliminary application in nonwoven fabric products.</p></div><div><h3>Results</h3><p>The alcoholic crude extracts of <em>C. sappan</em> heartwood consisted of alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, terpenoids, and cardiac glycosides. The <em>C. sappan</em> extract (CSE) had the highest inhibitory activity against <em>Staphylococcus aureus</em> with inhibition zone of 13.67 ± 1.56 mm in the agar well diffusion assay. The MIC value of CSE was at 1.95, 1.95, and 0.98 mg/ml, while the MBC value was 62.5, 3.91, and 31.25 mg/ml for <em>S. aureus</em>, <em>Bacillus cereus</em>, and <em>Vibrio parahaemolyticus,</em> respectively. The phenolic content was stable at 28 ± 2°C and at pH 4.0 for 12 h (∼95%) and 10 d (>90%), and it had low hemolytic activity on human erythrocytes at 5.04–18.95%. The major chemical component was flavonoids, and brazilein was 1.88% area sum. For the application to antibacterial fabrics, the highest inhibitory activity against <em>S. aureus</em> was found in 2MIC of CSE-coated fabric with 99% for 3 h.</p></div><div><h3>Conclusions</h3><p>The CSE had high inhibitory toward 3 bacterial strains and stability in a wide range of pH and temperature. The CSE-coated nonwoven fabric had efficient inhibition to <em>S. aureus</em> and a possibility to application in a nonwoven product in the future.</p><p><strong>How to cite:</strong> Hemthanon T, Ungcharoenwiwat P. Antibacterial activity, stability, and hemolytic activity of heartwood extract from <em>Caesalpinia sappan</em> for application on nonwoven fabric. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.10.002</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"55 ","pages":"Pages 9-17"},"PeriodicalIF":2.3000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S071734582100049X/pdfft?md5=4aeda7ed105c191a5226b4d551399ce3&pid=1-s2.0-S071734582100049X-main.pdf","citationCount":"10","resultStr":"{\"title\":\"Antibacterial activity, stability, and hemolytic activity of heartwood extract from Caesalpinia sappan for application on nonwoven fabric\",\"authors\":\"Tharathip Hemthanon, Pakpimol Ungcharoenwiwat\",\"doi\":\"10.1016/j.ejbt.2021.10.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Antimicrobial substances from medicinal plants have been widely applied in many industries, without the concern of side effects. Nonwoven fabric products such as face masks, paper wipes, and wound dressings contain antibacterial substances for reduction of bacterial accumulation. Therefore, the use of bioactive compounds from medicinal plants improves the properties of fabrics. This study used a crude extract from <em>Caesalpinia sappan</em> to perform phytochemical screening and investigate its antibacterial activity, stability, hemolytic activity, and preliminary application in nonwoven fabric products.</p></div><div><h3>Results</h3><p>The alcoholic crude extracts of <em>C. sappan</em> heartwood consisted of alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, terpenoids, and cardiac glycosides. The <em>C. sappan</em> extract (CSE) had the highest inhibitory activity against <em>Staphylococcus aureus</em> with inhibition zone of 13.67 ± 1.56 mm in the agar well diffusion assay. The MIC value of CSE was at 1.95, 1.95, and 0.98 mg/ml, while the MBC value was 62.5, 3.91, and 31.25 mg/ml for <em>S. aureus</em>, <em>Bacillus cereus</em>, and <em>Vibrio parahaemolyticus,</em> respectively. The phenolic content was stable at 28 ± 2°C and at pH 4.0 for 12 h (∼95%) and 10 d (>90%), and it had low hemolytic activity on human erythrocytes at 5.04–18.95%. The major chemical component was flavonoids, and brazilein was 1.88% area sum. For the application to antibacterial fabrics, the highest inhibitory activity against <em>S. aureus</em> was found in 2MIC of CSE-coated fabric with 99% for 3 h.</p></div><div><h3>Conclusions</h3><p>The CSE had high inhibitory toward 3 bacterial strains and stability in a wide range of pH and temperature. The CSE-coated nonwoven fabric had efficient inhibition to <em>S. aureus</em> and a possibility to application in a nonwoven product in the future.</p><p><strong>How to cite:</strong> Hemthanon T, Ungcharoenwiwat P. Antibacterial activity, stability, and hemolytic activity of heartwood extract from <em>Caesalpinia sappan</em> for application on nonwoven fabric. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.10.002</p></div>\",\"PeriodicalId\":11529,\"journal\":{\"name\":\"Electronic Journal of Biotechnology\",\"volume\":\"55 \",\"pages\":\"Pages 9-17\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S071734582100049X/pdfft?md5=4aeda7ed105c191a5226b4d551399ce3&pid=1-s2.0-S071734582100049X-main.pdf\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electronic Journal of Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S071734582100049X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S071734582100049X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 10
摘要
药用植物中的微生物物质在许多行业中得到了广泛的应用，并且没有副作用。非织造布产品，如口罩，纸巾和伤口敷料含有抗菌物质，以减少细菌的积累。因此，使用药用植物的生物活性化合物可以改善织物的性能。本研究以海麻粗提物为原料，进行植物化学筛选，考察其抗菌活性、稳定性、溶血活性，并初步探讨其在非织造布制品中的应用。结果菝葜心木醇性粗提物主要成分为生物碱、蒽醌类、香豆素类、黄酮类、皂苷类、单宁类、萜类和心苷类。在琼脂孔扩散实验中，菝葜提取物(CSE)对金黄色葡萄球菌的抑制活性最高，抑制区为13.67±1.56 mm。CSE的MIC值分别为1.95、1.95和0.98 mg/ml，金黄色葡萄球菌、蜡样芽孢杆菌和副溶血性弧菌的MBC值分别为62.5、3.91和31.25 mg/ml。在28±2°C和pH 4.0条件下，酚含量稳定12 h(~ 95%)和10 d (>90%)，对人红细胞的溶血活性较低，为5.04-18.95%。主要化学成分为黄酮类化合物，巴西豆素占总面积的1.88%。在抗菌织物的应用中，CSE涂层织物的2MIC对金黄色葡萄球菌的抑制活性最高，3 h的抑制率为99%。结论CSE对3种细菌具有较高的抑制作用，且在较宽的pH和温度范围内具有稳定性。该涂层非织造布对金黄色葡萄球菌具有良好的抑菌效果，在未来的非织造产品中具有广泛的应用前景。如何引用:Hemthanon T, Ungcharoenwiwat P. .杉木心材提取物在非织造布上的抗菌活性、稳定性和溶血活性。中国生物医学工程学报(英文版);2009;16。https://doi.org/10.1016/j.ejbt.2021.10.002
Antibacterial activity, stability, and hemolytic activity of heartwood extract from Caesalpinia sappan for application on nonwoven fabric
Background
Antimicrobial substances from medicinal plants have been widely applied in many industries, without the concern of side effects. Nonwoven fabric products such as face masks, paper wipes, and wound dressings contain antibacterial substances for reduction of bacterial accumulation. Therefore, the use of bioactive compounds from medicinal plants improves the properties of fabrics. This study used a crude extract from Caesalpinia sappan to perform phytochemical screening and investigate its antibacterial activity, stability, hemolytic activity, and preliminary application in nonwoven fabric products.
Results
The alcoholic crude extracts of C. sappan heartwood consisted of alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, terpenoids, and cardiac glycosides. The C. sappan extract (CSE) had the highest inhibitory activity against Staphylococcus aureus with inhibition zone of 13.67 ± 1.56 mm in the agar well diffusion assay. The MIC value of CSE was at 1.95, 1.95, and 0.98 mg/ml, while the MBC value was 62.5, 3.91, and 31.25 mg/ml for S. aureus, Bacillus cereus, and Vibrio parahaemolyticus, respectively. The phenolic content was stable at 28 ± 2°C and at pH 4.0 for 12 h (∼95%) and 10 d (>90%), and it had low hemolytic activity on human erythrocytes at 5.04–18.95%. The major chemical component was flavonoids, and brazilein was 1.88% area sum. For the application to antibacterial fabrics, the highest inhibitory activity against S. aureus was found in 2MIC of CSE-coated fabric with 99% for 3 h.
Conclusions
The CSE had high inhibitory toward 3 bacterial strains and stability in a wide range of pH and temperature. The CSE-coated nonwoven fabric had efficient inhibition to S. aureus and a possibility to application in a nonwoven product in the future.
How to cite: Hemthanon T, Ungcharoenwiwat P. Antibacterial activity, stability, and hemolytic activity of heartwood extract from Caesalpinia sappan for application on nonwoven fabric. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.10.002
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
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• Biopolicies of International Cooperation
• Biosafety
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• Biotechnology of Human Disorders
• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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