来自伊朗南部的破坏瓦螨(蜱螨亚纲:瓦螨总科)种群属于线粒体COI单倍型K

IF 0.7 Q4 ENTOMOLOGY

Persian Journal of Acarology Pub Date : 2019-04-15 DOI:10.22073/PJA.V8I2.43363

Zahra Hajializadeh, M. Asadi, K. Ahmadi, A. Balvasi

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引用次数: 0

摘要

利用线粒体细胞色素氧化酶亚基I基因的分子特征，首次对伊朗中南部种群进行了基因分型。用聚合酶链反应扩增出570 bp的片段，并用两种限制性内切酶进行酶切。用XhoI酶切的PCR产物在所有样品中都产生了300和270 bp的片段，而用SacI酶切的PCR产物没有产生任何酶切片段。结果表明，所有样本均属于先前文献中描述的破坏性v的K单倍型。在NCBI中首次登记到22个伊朗样本序列(418bp)。

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Varroa destructor (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI

A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.

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来源期刊

Persian Journal of Acarology Agricultural and Biological Sciences-Insect Science

CiteScore

1.60

自引率

30.80%

发文量

审稿时长

20 weeks

期刊介绍： Persian Journal of Acarology (PJA) is a peer-reviewed international journal of the Acarological Society of Iran for publication of high quality papers on any aspect of Acarology including mite and tick behavior, biochemistry, biology, control, ecology, evolution, morphology, physiology, systematics and taxonomy. All manuscripts will be subjected to peer review before acceptance.